不育男性的瘦素水平。

Leptin levels in infertile males.

作者信息

Jahan Sarwat, Bibi Riffat, Ahmed Shakeel, Kafeel Salma

机构信息

Department of Animal Sciences, Quaid-i-Azam University, Islamabad.

出版信息

J Coll Physicians Surg Pak. 2011 Jul;21(7):393-7.

PMID:21777525

Abstract

OBJECTIVE

To determine the leptin levels in the serum of normal, subfertile and infertile men.

STUDY DESIGN

Analytical study.

PLACE AND DURATION OF STUDY

Department of Animal Sciences Quaid-e-Azam University, Islamabad, National Institute of Health (NIH), Islamabad and Dr. Salma and Kafeel Medical Centre, Islamabad, from April to December 2009.

METHODOLOGY

Serum leptin levels hormonal concentrations (LH, FSH and testosterone) were determined by EIA in 154 males including 24 (15.58%) fertile, 19 (12.34%) polyzoospermic (PZs), 26 (16.88%) teratozoospermic (TZs), 27 (17.53%) astheno-teratozoospermic (ATZs), 18 (11.69%) oligozoospermic (OZs), 18 (11.69%) oligo-astheno-teratozoospermic (OATZs), 11 (7.14%) obstructive azoospermic (OBST-AZOOs) and 11 (7.14%) non-obstructive azoospermic (NON-OBSTAZOOs). BMI was also determined, divided into groups of greater than 24. Hormonal concentrations were compared by ANOVA and correlation was performed by using Graph pad prism version 5.

RESULTS

Significantly high levels of leptin concentrations were found in fertile (p < 0.001) as well as TZs, PZs, OZs, ATZs, (p < 0.001), OATZs (p < 0.01) and NON-OBST-AZOOs (p < 0.05) male subjects with BMI > 24 compared to fertile and infertile male patients with BMI < 24. Serum testosterone concentrations were significantly lower in PZs, OATZs, OZs, NON-OBST-AZOOs male patients with BMI < 24 compared to PZs (p < 0.05), OATZs, OZs (p < 0.01), OBST-AZOOs (p < 0.001) male patients with BMI > 24. Leptin showed a significant positive correlation with LH (p < 0.01) and FSH (p < 0.002) and a significant negative correlation with testosterone (p < 0.001).

CONCLUSION

Abnormal leptin level was significantly associated with fertility problems in males. Providing a link between leptin and reproduction factors contributing in control of testosterone and gonadotropins secretion in many aspects depending on fertility status in male subjects. BMI appears to have significant association with serum leptin levels.

摘要

目的

测定正常、亚生育和不育男性血清中的瘦素水平。

研究设计

分析性研究。

研究地点及时间

2009年4月至12月，在伊斯兰堡的奎德-阿扎姆大学动物科学系、伊斯兰堡的国家卫生研究所（NIH）以及伊斯兰堡的萨尔玛博士和卡菲尔医疗中心。

方法

采用酶免疫分析（EIA）法测定了154名男性的血清瘦素水平和激素浓度（促黄体生成素、促卵泡生成素和睾酮），其中包括24名（15.58%）可育男性、19名（12.34%）多精子症（PZs）男性、26名（16.88%）畸形精子症（TZs）男性、27名（17.53%）弱畸精子症（ATZs）男性、18名（11.69%）少精子症（OZs）男性、18名（11.69%）少弱畸精子症（OATZs）男性、11名（7.14%）梗阻性无精子症（OBST - AZOOs）男性和11名（7.14%）非梗阻性无精子症（NON - OBSTAZOOs）男性。还测定了体重指数（BMI），并将其分为大于24的组。采用方差分析比较激素浓度，并使用Graph pad prism 5版本进行相关性分析。

结果

与BMI < 24的可育和不育男性患者相比，BMI > 24的可育男性以及TZs、PZs、OZs、ATZs男性（p < 0.001）、OATZs男性（p < 0.01）和NON - OBST - AZOOs男性（p < 0.05）的血清瘦素浓度显著升高。与BMI > 24的PZs、OATZs、OZs、OBST - AZOOs男性患者相比，BMI < 24的PZs、OATZs、OZs、NON - OBST - AZOOs男性患者的血清睾酮浓度显著降低（PZs，p < 0.05；OATZs、OZs，p < 0.01；OBST - AZOOs，p < 0.001）。瘦素与促黄体生成素（p < 0.01）和促卵泡生成素（p < 0.002）呈显著正相关，与睾酮呈显著负相关（p < 0.001）。