Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Av. Antônio Carlos, 6627, Belo Horizonte, MG, Brazil.
Vet Microbiol. 2011 Dec 29;154(1-2):69-77. doi: 10.1016/j.vetmic.2011.06.027. Epub 2011 Jul 1.
Psittacid herpesvirus (PsHV) was isolated from 41 birds kept in captivity in Belo Horizonte, Minas Gerais/Brazil using chicken embryo fibroblasts (CEF) cell cultures. For this study, leukocytes or cloacal swabs of live birds were used. Also, portions of liver, spleen or kidney from birds collected at necropsy were utilized for these tests. PCR tests confirmed the presence of PsHV in 100% of samples. Thirty-three of the PCR products were sequenced and the results disclosed a 99% and 100% identity when compared with other sequences PsHV-1 (AY372243.1 and AF261756.1), previously deposited in GenBank. In addition, histopathology was performed and 19 of the 29 birds contained random multifocal lymphoplasmacytic hepatitis with necrotic foci, suggestive of viral infection. Three samples were examined by electron microscopy to visualize the viral particles obtained from cell culture. The viral structures measured 269 nm in average, had envelopes with an icosahedral capsid and tegument, consistent with herpesvirus. Thus, a total of 41 isolates were obtained from PsHV cell cultivation in CEF, confirming the circulation of the virus between parrots kept in captivity in Belo Horizonte, and affirming the importance of further studies in this area.
从巴西米纳斯吉拉斯州贝洛奥里藏特圈养的 41 只鸟中分离出了鹦鹉疱疹病毒（PsHV），使用鸡胚成纤维细胞（CEF）细胞培养。在这项研究中，使用了活鸟的白细胞或泄殖腔拭子。此外，还利用从剖检中收集的鸟类的肝脏、脾脏或肾脏部分进行了这些测试。PCR 测试证实了 100%的样本中存在 PsHV。对 33 个 PCR 产物进行测序，结果与先前在 GenBank 中存储的其他 PsHV-1（AY372243.1 和 AF261756.1）序列的同源性为 99%和 100%。此外，进行了组织病理学检查，29 只鸟中有 19 只随机出现多灶性淋巴浆细胞肝炎伴坏死灶，提示病毒感染。对 3 个样本进行了电子显微镜检查，以观察从细胞培养中获得的病毒颗粒。病毒结构的平均直径为 269nm，具有包膜，包含二十面体衣壳和衣壳，与疱疹病毒一致。因此，从 CEF 中的 PsHV 细胞培养中总共获得了 41 个分离株，证实了该病毒在贝洛奥里藏特圈养的鹦鹉之间的传播，并证实了在该领域进一步研究的重要性。
