College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Fish Shellfish Immunol. 2011 Dec;31(6):1224-31. doi: 10.1016/j.fsi.2011.07.011. Epub 2011 Jul 19.
In this study, we described the structure, mRNA tissue distribution and regulation of an IRF-3 gene from turbot, Scophthalmus maximus (SmIRF-3). The gene sequence of SmIRF-3 is 6077 bp long, composed of 11 exons and 10 introns similar to known IRF-3 genes of fish, and encodes a peptide of 466 amino acids. The deduced protein sequence shares the highest identity of 56.0-81.2% with fish IRF-3 and possesses a DNA-binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD) known to be important for the functions of IRF-3 in vertebrates. Phylogenetic analysis grouped SmIRF-3 with other IRF3s of vertebrates. SmIRF-3 transcripts were detectable in limited tissue types of healthy fish, with higher expression observed in head, kidney, spleen and kidney,. The SmIRF-3 was transcriptionally up-regulated by turbot reddish body iridovirus (TRBIV) and polyinosinic:polycytidylic acid (poly I:C) in the head kidney, spleen and gills, with showing a two wave induced expression during a 7-day time course in all cases. The highest inducibility and the likely earliest increase of SmIRF-3 expression were observed in the spleen, and poly I:C was a stronger inducer. In addition, the maximal expression level of SmIRF-3 arose prior to that of the Mx in all the cases.
在这项研究中，我们描述了大菱鲆（Scophthalmus maximus）IRF-3 基因的结构、mRNA 组织分布和调控。SmIRF-3 基因序列长 6077bp，由 11 个外显子和 10 个内含子组成，与已知鱼类的 IRF-3 基因相似，并编码一个由 466 个氨基酸组成的肽。推断的蛋白质序列与鱼类的 IRF-3 具有最高的同一性，为 56.0-81.2%，并具有已知对脊椎动物中 IRF-3 功能很重要的 DNA 结合域（DBD）、IRF 结合域（IAD）和富含丝氨酸的结构域（SRD）。系统进化分析将 SmIRF-3 与其他脊椎动物的 IRF3 分组在一起。SmIRF-3 转录本在健康鱼类的有限组织类型中可检测到，在头部、肾脏、脾脏和肾脏中表达较高。SmIRF-3 在头部肾脏、脾脏和鳃中受到虹彩病毒（TRBIV）和多聚肌苷酸：多聚胞苷酸（poly I:C）的转录上调，在所有情况下，在 7 天的时间过程中均显示出两波诱导表达。在所有情况下，SmIRF-3 的诱导能力最强，脾脏中的表达最早增加，而 poly I:C 是更强的诱导剂。此外，在所有情况下，SmIRF-3 的最大表达水平均先于 Mx 出现。
