Influence of age on noise- and styrene-induced hearing loss in the Long-Evans rat.

This paper reviews different investigations carried out with Long-Evans rats on the influence of age on the ototoxicity induced by styrene and on the vulnerability to noise. The first part of this article is focused on the differences in auditory susceptibility to noise (92 or 97dB octave band noise centered at 8kHz, 6 h/day, 5 days/week, 4 weeks) and styrene (700ppm, 6h/d, 5 d/w, 4 w) between young (three and half months) and old (24 months) Long-Evans rats. Auditory evoked potential measures revealed that the old rats tend to be more sensitive than young rats to higher noise levels (97dB), but equally vulnerable to moderate levels (92dB). By contrast, the aged rats were virtually insensitive to 700ppm styrene compared to the young animals. Two additional studies were performed controlling and examining the influence of body weight and post-natal age on the sensitivity to styrene. Rats of the same age (21 weeks) and but having different body weight (∼310g versus ∼410g) did not show any difference of sensitivity to 700ppm styrene, whereas 14-week-old rats with the same body weight as 21-week-old rats (∼350g) revealed increased sensitivity to styrene. These results show that weight does not play a key role in the sensitivity to styrene, and suggest a long period of increased sensitivity to styrene during the first months of life.