Departamento de Ciências Fisiológicas, Universidade Estadual de Londrina, C.P. 6001, 86051-990 Londrina, Paraná, Brazil.
Environ Toxicol Pharmacol. 2006 Jan;21(1):61-9. doi: 10.1016/j.etap.2005.07.016. Epub 2005 Oct 5.
The aims of the present study were to verify the suitability of short-term in situ test with the neotropical fish Prochilodus lineatus and to evaluate from a set of biochemical and physiological biomarkers the ones which could work as sensitivity tools for the environmental quality assessment. In situ tests were carried out for 1 week in winter and summer, at three sites along an urban stream heavily contaminated by anthropogenic activities and at a reference site. The variables analyzed were: hemoglobin content (Hb), plasma concentrations of cortisol, glucose, total protein, Na(+) and Cl(-), plasma osmolarity, liver activities of glutathione-S-transferase (GST) and catalase and interrenal cells area. Results showed that glycemia, interrenal cell size and GST activity, which were significantly higher in fish caged in the urban stream, were best able to distinguish between the most disturbed sites and the reference and caged P. lineatus showed to be a promising tool for the assessment and monitoring of tropical aquatic ecosystems.
本研究的目的是验证短期原位试验在新热带鱼类 Prochilodus lineatus 中的适用性，并从一系列生化和生理生物标志物中评估哪些标志物可用作环境质量评估的敏感工具。在冬季和夏季，分别在受人为活动严重污染的城市溪流的三个地点和一个参照点进行了为期一周的原位试验。分析的变量包括：血红蛋白含量 (Hb)、皮质醇、葡萄糖、总蛋白、Na(+)和 Cl(-)的血浆浓度、血浆渗透压、谷胱甘肽-S-转移酶 (GST)和过氧化氢酶的肝脏活性以及肾上腺细胞面积。结果表明，在城市溪流中饲养的鱼类的血糖、肾上腺细胞大小和 GST 活性显著升高，最能区分最受干扰的地点和参照点，并且笼养 P. lineatus 被证明是评估和监测热带水生生态系统的有前途的工具。
