两种需氧菌对偶氮二氯乙烯生物降解的动力学模型。

Kinetic models of dichloroethylene biodegradation by two strains of aerobic bacteria.

机构信息

School of Water Resource and Environment, China University of Geosciences, Beijing.

出版信息

Biomed Environ Sci. 2011 Jun;24(3):261-7. doi: 10.3967/0895-3988.2011.03.009.

DOI:10.3967/0895-3988.2011.03.009

PMID:21784312

Abstract

OBJECTIVE

In this study, we examined the biodegradation of Dichloroethylene (DCE) by two strains of aerobic bacteria.

METHODS

Using batch experiments, we measured the biodegradation rates of DCE and the residual concentrations of DCE for each bacterial strain. The varying trends in biodegradation rates with different initial concentrations of DCE were fitted to kinetic models.

RESULTS

The biodegradation kinetics of DCE by the strain DT-X, which uses toluene as co-metabolic substrate, fitted the Monod model (corresponding parameters: v(max)=0.0075 h(-1), K(s)=2.12 mg/L). The biodegradation kinetics of DCE by the strain DT-M, which uses 1,1-Dichloroethylene as single substrate, fitted the Haldane model (parameters: v(max) =0.0046 h(-1), K(s)=4.25 mg/L, K(i)=8.47 mg/L).

CONCLUSION

The substrate removal rate constant of 1,1-Dichloroethylene of the co-metabolic strain DT-X was much higher than that of strain DT-M. The substrate removal rates obtained from both bacterial strains in this study were higher than those reported in similar studies.

摘要

目的

本研究考察了两种需氧细菌对二氯乙烯（DCE）的生物降解作用。

方法

采用批式实验，我们测定了两种细菌对 DCE 的生物降解速率和 DCE 的残余浓度。用不同初始 DCE 浓度拟合生物降解速率的变化趋势，采用动力学模型进行拟合。

结果

以甲苯为共代谢基质的 DT-X 菌株对 DCE 的生物降解动力学符合 Monod 模型（相应参数：v(max)=0.0075 h(-1)，K(s)=2.12 mg/L）。以 1,1-二氯乙烯为单一基质的 DT-M 菌株对 DCE 的生物降解动力学符合 Haldane 模型（参数：v(max)=0.0046 h(-1)，K(s)=4.25 mg/L，K(i)=8.47 mg/L）。