B7-H3 在前列腺癌中的过表达促进肿瘤细胞进展。

B7-H3 over expression in prostate cancer promotes tumor cell progression.

机构信息

Department of Urology, First Affiliated Hospital of Soochow University and Institute of Medical Biotechnology, Soochow University, Suzhou, People's Republic of China.

出版信息

J Urol. 2011 Sep;186(3):1093-9. doi: 10.1016/j.juro.2011.04.103. Epub 2011 Jul 23.

DOI:10.1016/j.juro.2011.04.103

PMID:21784485

Abstract

PURPOSE

We compared B7-H3 expression in benign prostatic hyperplasia and prostate cancer tissue specimens, and determined the effects of low B7-H3 expression on the PC-3 human prostate cancer cell line using RNA interference.

MATERIALS AND METHODS

B7-H3 expression in prostate specimens was determined by enzyme-linked immunosorbent assay. A PC-3 cell line with low B7-H3 expression was established by RNA interference to investigate the effect of B7-H3 on cell proliferation, adhesion, migration and invasion in vitro.

RESULTS

B7-H3 in tissue samples was significantly higher in the prostate cancer group than in the benign prostatic hyperplasia group (mean±SEM 174.73±56.80 vs 82.69±46.19 ng/gm, p<0.001). B7-H3 expression down-regulated by small interfering RNA decreased cell adhesion to PC-3 fibronectin more than 30%, and migration and Matrigel™ invasion up to 50%. No apparent impact was observed on cell proliferation.

CONCLUSIONS

B7-H3 is aberrantly expressed in prostate cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating PC-3 cell progression.

摘要

目的

我们比较了良性前列腺增生和前列腺癌组织标本中的 B7-H3 表达，并通过 RNA 干扰确定低 B7-H3 表达对 PC-3 人前列腺癌细胞系的影响。

材料和方法

通过酶联免疫吸附试验确定前列腺标本中的 B7-H3 表达。通过 RNA 干扰建立低 B7-H3 表达的 PC-3 细胞系，以研究 B7-H3 对体外细胞增殖、黏附、迁移和侵袭的影响。

结果

组织样本中的 B7-H3 在前列腺癌组中明显高于良性前列腺增生组（平均值±SEM 174.73±56.80 与 82.69±46.19 ng/gm，p<0.001）。小干扰 RNA 下调的 B7-H3 表达使 PC-3 纤维连接蛋白上的细胞黏附减少了 30%以上，迁移和 Matrigel™侵袭增加了 50%。对细胞增殖没有明显影响。