Department of Urology, First Affiliated Hospital of Soochow University and Institute of Medical Biotechnology, Soochow University, Suzhou, People's Republic of China.
J Urol. 2011 Sep;186(3):1093-9. doi: 10.1016/j.juro.2011.04.103. Epub 2011 Jul 23.
We compared B7-H3 expression in benign prostatic hyperplasia and prostate cancer tissue specimens, and determined the effects of low B7-H3 expression on the PC-3 human prostate cancer cell line using RNA interference.
B7-H3 expression in prostate specimens was determined by enzyme-linked immunosorbent assay. A PC-3 cell line with low B7-H3 expression was established by RNA interference to investigate the effect of B7-H3 on cell proliferation, adhesion, migration and invasion in vitro.
B7-H3 in tissue samples was significantly higher in the prostate cancer group than in the benign prostatic hyperplasia group (mean±SEM 174.73±56.80 vs 82.69±46.19 ng/gm, p<0.001). B7-H3 expression down-regulated by small interfering RNA decreased cell adhesion to PC-3 fibronectin more than 30%, and migration and Matrigel™ invasion up to 50%. No apparent impact was observed on cell proliferation.
B7-H3 is aberrantly expressed in prostate cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating PC-3 cell progression.
我们比较了良性前列腺增生和前列腺癌组织标本中的 B7-H3 表达，并通过 RNA 干扰确定低 B7-H3 表达对 PC-3 人前列腺癌细胞系的影响。
通过酶联免疫吸附试验确定前列腺标本中的 B7-H3 表达。通过 RNA 干扰建立低 B7-H3 表达的 PC-3 细胞系，以研究 B7-H3 对体外细胞增殖、黏附、迁移和侵袭的影响。
组织样本中的 B7-H3 在前列腺癌组中明显高于良性前列腺增生组（平均值±SEM 174.73±56.80 与 82.69±46.19 ng/gm，p<0.001）。小干扰 RNA 下调的 B7-H3 表达使 PC-3 纤维连接蛋白上的细胞黏附减少了 30%以上，迁移和 Matrigel™侵袭增加了 50%。对细胞增殖没有明显影响。
B7-H3 在前列腺癌中异常表达。除了调节肿瘤免疫外，B7-H3 可能在调节 PC-3 细胞进展方面具有新的作用。
