物质 P 在丽纹石龙子（Hemidactylus flaviviridis）中的免疫调节作用：一项体外研究。
Immunomodulatory role of substance P in the wall lizard Hemidactylus flaviviridis: an in vitro study.
机构信息
Department of Zoology, University of Delhi, Delhi, India.
出版信息
Neuropeptides. 2011 Oct;45(5):323-8. doi: 10.1016/j.npep.2011.07.001. Epub 2011 Jul 23.
Present in vitro investigation for the first time in ectotherms demonstrated the immunomodulatory role of substance P in the wall lizard Hemidactylus flaviviridis. Substance P inhibited the percentage phagocytosis and phagocytic index of lizard splenic phagocytes. Inhibitory effect of substance P was completely blocked by NK-1 receptor antagonist spantide I, indicating the NK-1 receptor mediated action. Further, NK-1 receptor-coupled downstream signaling cascade involved in controlling phagocytosis was explored using inhibitors of adenylate cyclase (SQ 22536) and protein kinase A (H-89). Both the inhibitors, in a concentration-related manner decreased the suppressive effect of substance P on phagocytosis. In addition, substance P treatment caused an increase in intracellular cAMP level in splenic phagocytes. Taken together, it can be suggested that substance P via NK-1 receptor-coupled AC-cAMP-PKA pathway modulated the phagocytic activity of splenic phagocytes in wall lizards.
这是首次在变温动物中进行的体外研究，证明 P 物质在黄纹石龙子（Hemidactylus flaviviridis）中具有免疫调节作用。P 物质抑制了蜥蜴脾吞噬细胞的吞噬百分率和吞噬指数。P 物质的抑制作用被 NK-1 受体拮抗剂 Spantide I 完全阻断，表明 NK-1 受体介导的作用。此外，还使用环腺苷酸（SQ 22536）和蛋白激酶 A（H-89）抑制剂探索了与 NK-1 受体偶联的下游信号转导级联在控制吞噬作用中的作用。这两种抑制剂均以浓度相关的方式降低了 P 物质对吞噬作用的抑制作用。此外，P 物质处理导致脾吞噬细胞内 cAMP 水平升高。综上所述，可以认为 P 物质通过 NK-1 受体偶联的 AC-cAMP-PKA 途径调节了黄纹石龙子脾吞噬细胞的吞噬活性。
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