Bissell D M, Friedman S L, Maher J J, Roll F J
Liver Core Center, University of California, San Francisco General Hospital 94110.
Hepatology. 1990 Mar;11(3):488-98. doi: 10.1002/hep.1840110322.
The past 15 years have seen major advances in the characterization of extracellular matrix proteins and structure of matrix. As a by-product of this work, investigators now have an array of molecular and immunological reagents for monitoring matrix metabolism. Progress in the isolation and culture of individual cell types from liver has made possible direct measurement of matrix protein production and also has opened the way to studies of matrix degradation. The expanding knowledge of soluble mediators of inflammation is being applied to the regulation of matrix protein synthesis and degradation. Finally, experimental models of fibrosis in vivo are available for defining the complexity of matrix metabolism in the intact tissue and for validating the findings from cell culture and in vitro systems.
在过去的15年里，细胞外基质蛋白的特性及基质结构方面取得了重大进展。作为这项工作的一个副产品，研究人员现在有一系列用于监测基质代谢的分子和免疫试剂。从肝脏中分离和培养单个细胞类型的进展使得直接测量基质蛋白的产生成为可能，也为基质降解的研究开辟了道路。对炎症可溶性介质的认识不断扩展，正应用于基质蛋白合成和降解的调控。最后，体内纤维化的实验模型可用于确定完整组织中基质代谢的复杂性，并验证细胞培养和体外系统的研究结果。
