Sajewicz Mieczysław, Staszek Dorota, Natic Maja, Waksmundzka-Hajnos Monika, Kowalska Teresa
Institute of Chemistry, University of Silesia, 9 Szkolna Street, 40-006 Katowice, Poland.
J Chromatogr Sci. 2011 Aug;49(7):560-7. doi: 10.1093/chrsci/49.7.560.
In the previous paper from this series, we proposed mass spectrometric fingerprinting of complex botanical samples upon the examples of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. In this study, we explore fingerprinting efficiency with a novel two-dimensional analytical system composed of the reversed-phase thin-layer chromatography and the reversed-phase high performance liquid chromatography with mass spectrometric detection (2D RP-TLC-RP-LC-MS). We also compare its efficiency with that of the one-dimensional analytical system (the reversed-phase thin-layer chromatography with mass spectrometric detection; 1D RP-TLC-MS). As our present study is basically focused on the method development, we considered it as justified to carry out our comparison with the phenolic acid extracts selectively derived from the Salvia lavandulifolia species, similar as it was done in Part II from this series. Upon the results obtained, it was established that the 1D RP-TLC-MS mode and the 2D RP-TLC-RP-LC-MS mode can be applied to fingerprinting of herbal extracts, and that the 2D RP-TLC-RP-LC mode can provide a more abundant information than that originating from the 1D RP-TLC mode.
在本系列的上一篇论文中，我们以从西班牙鼠尾草中选择性提取的具有药理活性的酚酸和黄酮类化合物为例，提出了复杂植物样品的质谱指纹图谱法。在本研究中，我们使用由反相薄层色谱和反相高效液相色谱-质谱检测组成的新型二维分析系统（二维RP-TLC-RP-LC-MS）探索指纹图谱效率。我们还将其效率与一维分析系统（反相薄层色谱-质谱检测；一维RP-TLC-MS）进行比较。由于我们目前的研究主要集中在方法开发上，我们认为与从西班牙鼠尾草中选择性提取的酚酸提取物进行比较是合理的，就像本系列第二部分所做的那样。根据获得的结果，确定一维RP-TLC-MS模式和二维RP-TLC-RP-LC-MS模式可用于草药提取物的指纹图谱分析，并且二维RP-TLC-RP-LC模式比一维RP-TLC模式能提供更丰富的信息。
