当被整合到源自微管相关核蛋白的包涵体内时，IC 标记的蛋白质能够相互作用并进行复杂的反应。

IC-tagged proteins are able to interact with each other and perform complex reactions when integrated into muNS-derived inclusions.

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia y Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, Campus Vida S/N, 15782-Santiago de Compostela (A Coruña), Spain.

出版信息

J Biotechnol. 2011 Sep 20;155(3):284-6. doi: 10.1016/j.jbiotec.2011.07.010. Epub 2011 Jul 28.

DOI:10.1016/j.jbiotec.2011.07.010

PMID:21802457

Abstract

We have recently developed a versatile tagging system (IC-tagging) that causes relocation of the tagged proteins to ARV muNS-derived intracellular globular inclusions. In the present study we demonstrate (i) that the IC-tag can be successfully fused either to the amino or carboxyl terminus of the protein to be tagged and (ii) that IC-tagged proteins are able to interact between them and perform complex reactions that require such interactions while integrated into muNS inclusions, increasing the versatility of the IC-tagging system. Also, our studies with the DsRed protein add some light on the structure/function relationship of the evolution of DsRed chromophore.

摘要

我们最近开发了一种通用的标记系统（IC 标记），该系统可使标记蛋白重新定位到 ARV muNS 衍生的细胞内球形内含物。在本研究中，我们证明了（i）IC 标记可以成功融合到待标记蛋白的氨基或羧基末端，以及（ii）IC 标记蛋白能够相互作用，并在整合到 muNS 内含物时进行需要这种相互作用的复杂反应，从而增加了 IC 标记系统的多功能性。此外，我们对 DsRed 蛋白的研究也揭示了 DsRed 生色团进化的结构/功能关系。