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通过二维T(2)-T(2)相关核磁共振光谱法测量弹性蛋白中水合水的交换率

Measurement of the Exchange Rate of Waters of Hydration in Elastin by 2D T(2)-T(2) Correlation Nuclear Magnetic Resonance Spectroscopy.

作者信息

Sun Cheng, Boutis Gregory S

机构信息

Brooklyn College, Department of Physics 2900 Bedford Avenue Brooklyn NY 11210.

出版信息

New J Phys. 2011 Feb 28;13:2-16. doi: 10.1088/1367-2630/13/2/025026.

DOI:10.1088/1367-2630/13/2/025026
PMID:21804764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3144479/
Abstract

We report on the direct measurement of the exchange rate of waters of hydration in elastin by T(2)-T(2) exchange spectroscopy. The exchange rates in bovine nuchal ligament elastin and aortic elastin at temperatures near, below and at the physiological temperature are reported. Using an Inverse Laplace Transform (ILT) algorithm, we are able to identify four components in the relaxation times. While three of the components are in good agreement with previous measurements that used multi-exponential fitting, the ILT algorithm distinguishes a fourth component having relaxation times close to that of free water and is identified as water between fibers. With the aid of scanning electron microscopy, a model is proposed allowing for the application of a two-site exchange analysis between any two components for the determination of exchange rates between reservoirs. The results of the measurements support a model (described elsewhere [1]) wherein the net entropy of bulk waters of hydration should increase upon increasing temperature in the inverse temperature transition.

摘要

我们报告了通过T(2)-T(2)交换光谱法对弹性蛋白中水合水交换率的直接测量。报告了牛颈部韧带弹性蛋白和主动脉弹性蛋白在接近、低于和生理温度下的交换率。使用逆拉普拉斯变换（ILT）算法，我们能够在弛豫时间中识别出四个成分。虽然其中三个成分与之前使用多指数拟合的测量结果高度一致，但ILT算法区分出了第四个成分，其弛豫时间与自由水相近，被确定为纤维间的水。借助扫描电子显微镜，提出了一个模型，允许在任意两个成分之间应用两点交换分析来确定储库之间的交换率。测量结果支持了一个模型（在其他地方有描述[1]），即在逆温度转变中，随着温度升高，大量水合水的净熵应该增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/2e6f25fef164/nihms308421f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/a7a8d70a6e07/nihms308421f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/62f4b0a84340/nihms308421f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/6a1bc8794056/nihms308421f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/55989c81301d/nihms308421f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/b20d5f78cb56/nihms308421f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/8e41d4709c8b/nihms308421f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/0921e04f6213/nihms308421f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/b79f0fc0b6e6/nihms308421f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/2e6f25fef164/nihms308421f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/a7a8d70a6e07/nihms308421f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/62f4b0a84340/nihms308421f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/6a1bc8794056/nihms308421f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/55989c81301d/nihms308421f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/b20d5f78cb56/nihms308421f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/8e41d4709c8b/nihms308421f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/0921e04f6213/nihms308421f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/b79f0fc0b6e6/nihms308421f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4109/3144479/2e6f25fef164/nihms308421f9.jpg

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