Suppr超能文献

用8-叠氮基-dATP对克列诺片段进行光亲和标记。

Photoaffinity labeling of the Klenow fragment with 8-azido-dATP.

作者信息

Rush J, Konigsberg W H

机构信息

Department of Molecular Biophysics and Biochemistry, Yale School of Medicine, New Haven, Connecticut 06510.

出版信息

J Biol Chem. 1990 Mar 25;265(9):4821-7.

PMID:2180951
Abstract

The photoaffinity compound 8-azido-dATP was used as a probe for the deoxyribonucleoside triphosphate-binding site of the large fragment of DNA polymerase I. Azido-dATP specifically modified a saturable binding site within the Klenow fragment, and each of the four natural deoxyribonucleoside triphosphate substrates competed with labeling at this site in proportion to its binding constant, as previously defined by equilibrium dialysis. Analysis of tryptic peptides after azido-dATP modification revealed five major cross-linking products, which apparently arose from five distinct photoadducts formed near Tyr-766.

摘要

光亲和化合物8-叠氮基-dATP被用作DNA聚合酶I大片段的脱氧核糖核苷三磷酸结合位点的探针。叠氮基-dATP特异性修饰了Klenow片段内的一个可饱和结合位点,并且四种天然脱氧核糖核苷三磷酸底物中的每一种都与该位点的标记竞争,其竞争比例与其结合常数成正比,如先前通过平衡透析所定义的那样。叠氮基-dATP修饰后胰蛋白酶肽段的分析揭示了五种主要的交联产物,它们显然来自于在Tyr-766附近形成的五种不同的光加合物。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验