原儿茶酸对新生大鼠培养大脑皮质神经元轴突生长和存活的神经营养作用
[Neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons of newborn rat].
作者信息
Xue Xiao-yan, Lin Lian-feng, Xiao Fei, Pi Ting, Lai Yong-chang, Luo Huan-min
机构信息
School of Medicine, Jinan University, Guangzhou 510632, China.
出版信息
Zhong Yao Cai. 2011 Apr;34(4):567-72.
OBJECTIVE
To study the neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons.
METHODS
The newborn rat cerebral cortex neurons were cultured in vitro. The convert phase microscope was used to count the survival neurons with neurites and measure the average length of neurites. MTT and LDH assay were carried out to investigate the effect of PCA on the neuronal viability.
RESULTS
Compared with control group, different concentration of PCA could increase the number of survival neurons with neurites and the average length of neurites. MTT and LDH assay showed that PCA promoted neuron survival in a dose-dependent manner.
CONCLUSION
PCA can enhance the survival of rat cortical neurons with neurites and promote the neurite outgrowth of rat cortical neurons.
目的
研究原儿茶酸对体外培养的大脑皮质神经元轴突生长及存活的神经营养作用。
方法
体外培养新生大鼠大脑皮质神经元，用倒置相差显微镜计数有轴突的存活神经元数量并测量轴突平均长度，采用MTT法和乳酸脱氢酶（LDH）测定法研究原儿茶酸对神经元活力的影响。
结果
与对照组相比，不同浓度的原儿茶酸均可增加有轴突的存活神经元数量及轴突平均长度。MTT法和LDH测定法显示原儿茶酸以剂量依赖方式促进神经元存活。
结论
原儿茶酸可提高大鼠有轴突皮质神经元的存活率并促进大鼠皮质神经元轴突生长。
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