对一种对细胞转化温度敏感的劳氏肉瘤病毒突变体的基因组RNA的特性分析。
Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation.
作者信息
Darlix J L, Levray M, Bromley P A, Spahr P F
出版信息
Nucleic Acids Res. 1979 Feb;6(2):471-85. doi: 10.1093/nar/6.2.471.
We have found that the LA23 t/s mutant of Rous sarcoma virus (phenotype Prague B), even when passaged repeatedly at high multiplicity of infection, does not give rise to transformation defective deletion mutants comparable to those derived from RSV. In view of this fact and of the high rate of production of this mutant at 41 degrees C, we have undertaken a detailed analysis of the genome of this virus by ordering all large T1 oligonucleotides and by determining their nucleotide sequences. The results indicate a high degree of mutation in the onc gene as compared to that of Pr-A or Pr-B.
我们发现，劳氏肉瘤病毒的LA23 t/s突变体（表型为布拉格B），即使在高感染复数下反复传代，也不会产生与源自劳氏肉瘤病毒的那些转化缺陷型缺失突变体相当的突变体。鉴于这一事实以及该突变体在41摄氏度时的高产生率，我们通过对所有大的T1寡核苷酸进行排序并确定其核苷酸序列，对该病毒的基因组进行了详细分析。结果表明，与Pr-A或Pr-B相比，癌基因中的突变程度很高。
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