Meng Lingzhu, Li Ming, Yang Seung Hwan, Kim Tae-Jong, Suh Joo-Won
Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Republic of Korea.
Biosci Biotechnol Biochem. 2011;75(8):1576-81. doi: 10.1271/bbb.110277. Epub 2011 Aug 7.
The addition of extracellular ATP (exATP) to four Streptomyces strains had similar effects: low exATP levels stimulated antibiotic production and high levels reduced it. Compared with antibiotic production, the concentrations of intracellular ATP (inATP) in the tested strains were opposite, which suggests a role of inATP in regulating secondary metabolite production. Under inactivation of the polyphosphate kinase gene (ppk) in Streptomyces lividans, we observed the same results: when the inATP level in the mutant strain was lower than in the parent strain, more antibiotic was produced. Combining all the results, a strong inverse relationship between [inATP] and the secondary metabolite production is suggested by this study.
向四种链霉菌菌株中添加细胞外ATP（exATP）具有相似的效果：低水平的exATP刺激抗生素产生，而高水平则抑制抗生素产生。与抗生素产生情况相比，受试菌株中细胞内ATP（inATP）的浓度呈现相反趋势，这表明inATP在调节次级代谢产物产生中发挥作用。在变铅青链霉菌的多聚磷酸激酶基因（ppk）失活的情况下，我们观察到了相同的结果：当突变菌株中的inATP水平低于亲本菌株时，产生的抗生素更多。综合所有结果，本研究表明[inATP]与次级代谢产物产生之间存在强烈的负相关关系。
