Grozdanova A, Poceva-Panovska A, Brezovska K, Trajkovska-Dokic E, Dimovski A, Apostolski S, Suturkova Lj
Pharmaceutical Chemistry Institute, Pharmacy Faculty,Ss Cyril and Methodius University, Skopje, R. Macedonia.
Prilozi. 2011;32(1):113-25.
Campylobacter jejuni (C. jejuni) infection frequently triggers autoimmune-mediated neuropathies, especially the Guillain-Barre syndrome (GBS). The molecular mimicry between the core oligosaccharides of bacterial lipopolysaccharides (LPSs) and the human gangliosides presumably results in the production of anti-neural cross-reactive antibodies which are likely to be a contributory factor in the induction and pathogenesis of GBS. The aim of our study was to determine the presence of cross-reactive epitopes in C. jejuni LPSs isolated from enteritis patients and to determine their antigen reactivity. For that purpose we collected stool specimens from 21 patients with enteritis and without neurological symptoms. Seven different serotypes of C. jejuni (0:27; 0:6/0:7; 0:38; 0:3; 0:1/0:44; 0:19; 0:37) were detected using the Penner system. Unexpectedly, one serotype from this group was detected as 0:19, a serotype rarely isolated from enteritis patient and in close association with GBS. Binding studies using cholera toxin-B subunit and peanut agglutinin, showed the presence of ganglioside-like epitopes in C. jejuni strains 0:37, 0:19 and 0:27. Reactivity with sera from patient with GBS, with confirmed previous exposure to C. jejuni and with high a titre of anti-ganglioside antibodies, showed that the same three LPSs from C. jejuni serotypes 0:37, 0:19 and 0:27 bear cross-reactive epitopes in their LPSs structures. Our results confirm the results from previous studies that LPSs from certain C. jejuni serotypes bear cross-reactive ganglioside-like epitopes which might be involved in the induction of GBS after C. jejuni infection.
空肠弯曲菌（C. jejuni）感染常引发自身免疫介导的神经病变，尤其是吉兰 - 巴雷综合征（GBS）。细菌脂多糖（LPSs）的核心寡糖与人神经节苷脂之间的分子模拟大概会导致抗神经交叉反应性抗体的产生，而这些抗体很可能是GBS诱导和发病机制中的一个促成因素。我们研究的目的是确定从肠炎患者分离出的空肠弯曲菌LPSs中交叉反应性表位的存在，并确定它们的抗原反应性。为此，我们收集了21例无神经症状的肠炎患者的粪便标本。使用彭纳系统检测到7种不同血清型的空肠弯曲菌（0:27；0:6/0:7；0:38；0:3；0:1/0:44；0:19；0:37）。出乎意料的是，该组中的一种血清型被检测为0:19，这是一种很少从肠炎患者中分离出来且与GBS密切相关的血清型。使用霍乱毒素B亚基和花生凝集素进行的结合研究表明，空肠弯曲菌菌株0:37、0:19和0:27中存在神经节苷脂样表位。与先前确诊感染过空肠弯曲菌且抗神经节苷脂抗体滴度高的GBS患者血清的反应性表明，空肠弯曲菌血清型0:37、0:19和0:27的相同三种LPSs在其LPSs结构中带有交叉反应性表位。我们的结果证实了先前研究的结果，即某些空肠弯曲菌血清型的LPSs带有交叉反应性神经节苷脂样表位，这可能与空肠弯曲菌感染后GBS的诱导有关。
