Wang Xian-zhen, Jiang Jia-ye, Luo Shan-shan
Experimental Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Jun;31(6):811-5.
To study the vascular endothelial function recovery and its mechanism of Banxia Baizhu Tianma Decoction (BBTD).
54 SH rats were randomly divided into three groups: the blank control group, BBTD group and Captopril treatment group. BBTD (at the daily dose of 4. 320 g crude drug/kg) and Captopril (at the daily dose of 3.375 g/kg) was administered from the 7th week to the 24th week. Another eighteen Wistar-Kyoto rats of the same ages were taken as the control. Medication was discontinued and effects were observed until the 32nd week. The blood pressure was determined by arterial carotis cannula. The concentration of serum NO2(-) and total anti-oxidation were determined by Griess and fluorescence recovery after photobleaching (FRAP). The acetylcholine (Ach)-dependent relaxation of superior mesenteric artery was detected using in vitro vascular ring. The mRNA expressions of IL-1, IL-6 and iNOS were detected by Real-time PCR at the 18th, 24th, and 32nd week.
BBTD could significantly lower blood pressure of SHR and the concentration of serum NO2(-) at the 18th and 24th week (P<0.05). The total anti-oxidation of SH rats increased at the 18th week (P<0.01), and ACh-dependent relaxation of superior mesenteric artery increased at the 24th week. The mRNA expressions of IL-1 was markedly suppressed by BBTD at the 18th, 24th, and 32nd week (P<0.05), while IL-6 and iNOS mRNA expression were significantly lowered only at the 32nd week (P< 0.01). Captopril could significantly lower blood pressure of SHR at the 18th and 24th week (P<0.05). It significantly increased the total anti-oxidation of SH rats at the 18th week (P<0.01). However, it could not increase ACh-dependent relaxation of superior mesenteric artery and regulate the concentration of NO2(-) at the 18th, 24th, and 32nd week. The mRNA expression of iNOS was markedly suppressed by Captopril at the 24th and 32nd week, while mRNA expressions of IL-1 and IL-6 were significantly lower only at the 32nd week (P<0.01).
BBTD showed similar effect in decreasing the blood pressure to captopril, but it showed better effect in improving the mesenteric endothelial dysfunction of SHR, which may be associated with its inhibition on NO and IL-1 expression, and improvement of the oxidative stress state.
研究半夏白术天麻汤（BBTD）对血管内皮功能的恢复作用及其机制。
将54只SH大鼠随机分为三组：空白对照组、BBTD组和卡托普利治疗组。从第7周开始至第24周，分别给予BBTD（每日剂量为4.320 g生药/kg）和卡托普利（每日剂量为3.375 g/kg）。另取18只同年龄的Wistar-Kyoto大鼠作为对照。停药后观察至第32周。通过颈动脉插管测定血压。采用Griess法和光漂白后荧光恢复法（FRAP）测定血清NO2(-)浓度和总抗氧化能力。采用离体血管环检测肠系膜上动脉乙酰胆碱（Ach）依赖性舒张功能。在第18、24和32周，通过实时PCR检测IL-1、IL-6和iNOS的mRNA表达。
BBTD可显著降低SHR在第18和24周时的血压以及血清NO2(-)浓度（P<0.05）。SH大鼠的总抗氧化能力在第18周时升高（P<0.01），肠系膜上动脉的Ach依赖性舒张功能在第24周时增强。BBTD在第18、24和32周时显著抑制IL-1的mRNA表达（P<0.05），而IL-6和iNOS的mRNA表达仅在第32周时显著降低（P<0.01）。卡托普利可在第18和24周时显著降低SHR的血压（P<0.05）。它在第18周时显著提高SH大鼠的总抗氧化能力（P<0.01）。然而，在第18、24和32周时，它不能增强肠系膜上动脉的Ach依赖性舒张功能，也不能调节NO2(-)浓度。卡托普利在第24和32周时显著抑制iNOS的mRNA表达，而IL-1和IL-6的mRNA表达仅在第32周时显著降低（P<0.01）。
BBTD在降低血压方面显示出与卡托普利相似的效果，但在改善SHR的肠系膜内皮功能障碍方面效果更好，这可能与其抑制NO和IL-1表达以及改善氧化应激状态有关。
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