Yang Yun-fan, Sun Qian, Su Bin-tao, Lin Lin, Li Man-jun, Chen Lin, Xu Hong, Lei Jia-hui, Liu Wen-qi, Li Yong-long
Second Clinical School, Tongi Medical College, Huarzhong University of Science and Technology, Wuhan 430030, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2011 Apr 30;29(2):101-4.
To investigate the effect of ageing on the immune responses against Schistosoma japonicum infection in mice.
Female BALB/c mice were divided into young group (2 months) and old group (18 months), each composed of 8 mice. Each mouse was percutaneously infected with 40 +/- 1 S. japonicum cercariae. At 6 weeks post-infection, the mice were sacrificed, and the spleens were removed and single-cell suspensions of splenocytes were prepared. Worms were perfused from hepatic portal system and counted. The number of eggs in the liver was determined after KOH digestion. Mean single-egg granulomas sizes were determined in stained histological sections. Splenocyte proliferation responses were analyzed by MTT colorimetry. Level of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in the splenocyte culture supernatants was determined by ELISA.
The worm burden and egg per gram of liver in old mice [19.75 +/- 1.95, (1.59 +/- 1.05) x 10(4)] were significantly lower than that of young mice [26.00 +/- 2.42, (208 +/- 0.87) x 10(4)] (P < 0.05). The mean volume of single-egg granulomas of the livers in old mice [(30.13 +/- 10.97) x 10(3) mm3] was significantly lower than that of the young mice [(47.02 +/- 24.13) x l0(3) mm3] (P < 0.05).
of T cell proliferation showed that the splenocytes had poorer immune reactivity to ConA in old mice (SI: 1.08 +/- 0.12) than that in young mice (SI: 131 +/- 0.14) (P < 0.05). Levels of IFN-gamma and IL-4 in the splenocyte culture supernatants [(24.05 +/- 6.24), (4.15 +/- 0.68) pg/ml] from old mice were lower than that of young mice [(34.25 +/- 869), (7125 +/- 0.83) pg/ml](P < 0.05).
Ageing down-modulates the immune responses and the poorer immune reactivity might decrease pathological alterations in mice infected with Schistosoma japonicum.
研究衰老对小鼠日本血吸虫感染免疫反应的影响。
将雌性BALB/c小鼠分为青年组（2个月）和老年组（18个月），每组8只。每只小鼠经皮感染40±1条日本血吸虫尾蚴。感染后6周，处死小鼠，取出脾脏并制备脾细胞单细胞悬液。从肝门静脉系统灌注虫体并计数。用氢氧化钾消化后测定肝脏中的虫卵数。在染色组织切片中测定单个虫卵肉芽肿的平均大小。采用MTT比色法分析脾细胞增殖反应。用ELISA法测定脾细胞培养上清液中干扰素-γ（IFN-γ）和白细胞介素-4（IL-4）的水平。
老年小鼠的虫负荷和每克肝脏虫卵数[19.75±1.95，（1.59±1.05）×10⁴]显著低于青年小鼠[26.00±2.42，（208±0.87）×10⁴]（P<0.05）。老年小鼠肝脏单个虫卵肉芽肿的平均体积[（30.13±10.97）×10³mm³]显著低于青年小鼠[（47.02±24.13）×10³mm³]（P<0.05）。
T细胞增殖结果显示，老年小鼠脾细胞对刀豆蛋白A的免疫反应性（SI：1.08±0.12）低于青年小鼠（SI：131±0.14）（P<0.05）。老年小鼠脾细胞培养上清液中IFN-γ和IL-4的水平[（24.05±6.24），（4.15±0.68）pg/ml]低于青年小鼠[（34.25±869），（7125±0.83）pg/ml]（P<0.05）。
衰老下调免疫反应，免疫反应性降低可能会减少日本血吸虫感染小鼠的病理改变。
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