Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
J Med Virol. 2011 Oct;83(10):1783-91. doi: 10.1002/jmv.22198.
Enterovirus 71 (EV71) infection may cause severe neurological complications, particularly in young children. Despite the risks, there are still no commercially available EV71 vaccines. Hence, a candidate vaccine construct, containing recombinant Newcastle disease virus capsids that display an EV71 VP1 fragment (NPt-VP1(1-100) ) protein, was evaluated in a mouse model of EV71 infection. Previously, it was shown that this protein construct provoked a strong immune response in vaccinated adult rabbits. That study, however, did not address the issue of its effectiveness against EV71 infection in young animals. In the present study, EV71 viral challenge in vaccinated newborn mice resulted in more than 40% increase in survival rate. Significantly, half of the surviving mice fully recovered from their paralysis. Histological analysis of all of the surviving mice revealed a complete clearance of EV71 viral antigens from their brains and spinal cords. In hind limb muscles, the amounts of the antigens detected correlated with the degrees of tissue damage and paralysis. Findings from this study provide evidence that immunization with the NPt-VP1(1-100) immunogen in a newborn mouse model confers partial protection against EV71 infection, and also highlights the importance of NPt-VP1(1-100) as a possible candidate vaccine for protection against EV71 infections.
肠道病毒 71 型（EV71）感染可能导致严重的神经并发症，尤其是在幼儿中。尽管存在风险，但目前仍没有市售的 EV71 疫苗。因此，我们评估了一种含有重组新城疫病毒衣壳的候选疫苗构建体，该衣壳展示了 EV71 VP1 片段（NPt-VP1(1-100)）蛋白，在 EV71 感染的小鼠模型中进行了评估。先前的研究表明，这种蛋白构建体在接种的成年兔中引发了强烈的免疫反应。然而，该研究并未解决其在幼小动物中对 EV71 感染的有效性问题。在本研究中，接种疫苗的新生小鼠在 EV71 病毒攻击下的存活率提高了 40%以上。值得注意的是，一半的幸存小鼠完全从瘫痪中恢复。对所有幸存小鼠进行的组织学分析显示，它们的大脑和脊髓中的 EV71 病毒抗原已完全清除。在后肢肌肉中，检测到的抗原量与组织损伤和瘫痪的程度相关。这项研究的结果提供了证据，证明在新生小鼠模型中用 NPt-VP1(1-100)免疫原进行免疫接种可提供针对 EV71 感染的部分保护，并且还强调了 NPt-VP1(1-100)作为针对 EV71 感染的潜在候选疫苗的重要性。
