Department of Organic Chemistry, University of Geneva, Geneva, Switzerland.
Org Biomol Chem. 2011 Oct 7;9(19):6623-8. doi: 10.1039/c1ob05835e. Epub 2011 Aug 17.
Transport across the membranes of polymersomes remains difficult in part due to the great thickness of the polymer bilayers. Here, we report that dynamic polyion-counterion transport systems are active in fluorogenic polymersomes composed of poly(dimethylsiloxane)-b-poly(2-methyloxazoline) (PDMS-PMOXA). These results suggest that counterion-activated calf-thymus DNA can act as cation carrier that moves not only across lipid bilayer and bulk chloroform membranes but also across the "plastic" membranes of polymersomes. Compared to egg yolk phosophatidylcholine (EYPC) lipsosomes, activities and activator scope in PDMS-PMOXA polymersomes are clearly reduced. Embedded in agar gel matrices, fluorogenic PDMS-PMOXA polymersomes respond reliably to polyion-counterion transporters, with high contrast, high stability and preserved selectivity. Compared to standard EYPC liposomes, it cannot be said that PDMS-PMOXA polymersomes are better. However, they are different, and this difference could be interesting for the development of sensing devices.
聚合物囊泡的跨膜转运仍然很困难，部分原因是聚合物双层的厚度很大。在这里，我们报告说，在由聚（二甲基硅氧烷）-b-聚（2-甲基恶唑啉）（PDMS-PMOXA）组成的荧光聚合物囊泡中，动态聚离子抗衡离子转运系统是活跃的。这些结果表明，抗衡离子激活的小牛胸腺 DNA 可以作为阳离子载体，不仅可以穿过脂质双层和氯仿本体膜，还可以穿过聚合物囊泡的“塑料”膜。与蛋黄磷酯酰胆碱（EYPC）脂质体相比，PDMS-PMOXA 聚合物囊泡中的活性和激活剂范围明显降低。嵌入琼脂凝胶基质中，荧光 PDMS-PMOXA 聚合物囊泡对聚离子抗衡离子转运体可靠响应，具有高对比度、高稳定性和保留选择性。与标准的 EYPC 脂质体相比，不能说 PDMS-PMOXA 聚合物囊泡更好。然而，它们是不同的，这种差异可能对传感设备的发展很有趣。
