Williams D A
Howard Hughes Medical Institute, Children's Hospital, Boston, MA.
Bone Marrow Transplant. 1990 Mar;5(3):141-4.
Recent advances in the ability to introduce exogenous DNA sequences into specific chromosomal locations in mammalian cells via homologous recombination together with effective manipulation of murine embryos and embryo-derived cell lines (ES cells) make possible the generation of mouse stains with specific genes disrupted. Such gene targeting methods will allow replacement of normally functioning murine genes with genes altered with precise mutations involving many aspects of gene expression, including transcription, processing and protein-protein interactions. ES cell technology will allow experimental hematologists to produce animal models for human hematopoietic diseases and explore the role of complex proteins involved in hematopoiesis in vivo.
近期，通过同源重组将外源DNA序列导入哺乳动物细胞特定染色体位置的能力取得了进展，同时对小鼠胚胎和胚胎衍生细胞系（胚胎干细胞）进行有效操控，使得生成特定基因被破坏的小鼠品系成为可能。此类基因靶向方法将能够用包含基因表达多个方面（包括转录、加工及蛋白质-蛋白质相互作用）精确突变的基因，替代正常发挥功能的小鼠基因。胚胎干细胞技术将使实验血液学家能够建立人类血液疾病的动物模型，并在体内探索参与造血过程的复杂蛋白质的作用。
