National Animal TSE Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, People's Republic of China.
Ir Vet J. 2006 Mar 1;59(3):148-52. doi: 10.1186/2046-0481-59-3-148.
This is the first reported isolation of avian influenza virus (AIV) from emu in China. An outbreak of AIV infection occurred at an emu farm that housed 40 four-month-old birds. Various degrees of haemorrhage were discovered in the tissues of affected emus. Cell degeneration and necrosis were observed microscopically. Electron microscopy revealed round or oval virions with a diameter of 80 nm to 120 nm, surrounded by an envelope with spikes. The virus was classified as low pathogenic AIV (LPAIV), according to OIE standards. It was named A/Emu/HeNen/14/2004(H9N2)(Emu/HN/2004). The HA gene (1683bp) was amplified by RT-PCR and it was compared with other animal H9N2 AIV sequences in GenBank, the US National Institutes of Health genetic sequence database. The results suggested that Emu/HN/2004 may have come from an avian influenza virus (H9N2) from Southern China.
这是中国首次从鸸鹋中分离出禽流感病毒(AIV)。一起 AIV 感染疫情发生在一个饲养了 40 只四个月大鸸鹋的鸸鹋养殖场。受感染的鸸鹋的组织中发现了不同程度的出血。显微镜下观察到细胞变性和坏死。电子显微镜显示,病毒粒子呈圆形或椭圆形,直径为 80nm 至 120nm,周围有带有刺突的包膜。根据世界动物卫生组织(OIE)标准,该病毒被归类为低致病性禽流感病毒(LPAIV)。它被命名为 A/Emu/HeNen/14/2004(H9N2)(Emu/HN/2004)。通过 RT-PCR 扩增了 HA 基因(1683bp),并与美国国立卫生研究院遗传序列数据库(GenBank)中的其他动物 H9N2 AIV 序列进行了比较。结果表明,Emu/HN/2004 可能来自中国南方的禽流感病毒(H9N2)。