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从中国鸸鹋中分离出禽流感病毒(H9N2)。

Isolation of avian influenza virus (H9N2) from emu in China.

机构信息

National Animal TSE Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, People's Republic of China.

出版信息

Ir Vet J. 2006 Mar 1;59(3):148-52. doi: 10.1186/2046-0481-59-3-148.

Abstract

This is the first reported isolation of avian influenza virus (AIV) from emu in China. An outbreak of AIV infection occurred at an emu farm that housed 40 four-month-old birds. Various degrees of haemorrhage were discovered in the tissues of affected emus. Cell degeneration and necrosis were observed microscopically. Electron microscopy revealed round or oval virions with a diameter of 80 nm to 120 nm, surrounded by an envelope with spikes. The virus was classified as low pathogenic AIV (LPAIV), according to OIE standards. It was named A/Emu/HeNen/14/2004(H9N2)(Emu/HN/2004). The HA gene (1683bp) was amplified by RT-PCR and it was compared with other animal H9N2 AIV sequences in GenBank, the US National Institutes of Health genetic sequence database. The results suggested that Emu/HN/2004 may have come from an avian influenza virus (H9N2) from Southern China.

摘要

这是中国首次从鸸鹋中分离出禽流感病毒(AIV)。一起 AIV 感染疫情发生在一个饲养了 40 只四个月大鸸鹋的鸸鹋养殖场。受感染的鸸鹋的组织中发现了不同程度的出血。显微镜下观察到细胞变性和坏死。电子显微镜显示,病毒粒子呈圆形或椭圆形,直径为 80nm 至 120nm,周围有带有刺突的包膜。根据世界动物卫生组织(OIE)标准,该病毒被归类为低致病性禽流感病毒(LPAIV)。它被命名为 A/Emu/HeNen/14/2004(H9N2)(Emu/HN/2004)。通过 RT-PCR 扩增了 HA 基因(1683bp),并与美国国立卫生研究院遗传序列数据库(GenBank)中的其他动物 H9N2 AIV 序列进行了比较。结果表明,Emu/HN/2004 可能来自中国南方的禽流感病毒(H9N2)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66b2/3113888/52f5aabfc13a/2046-0481-59-3-148-1.jpg

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