Virus Research and Development, Department of Virology, Statens Serum Institut, Copenhagen, Denmark.
PLoS One. 2011;6(8):e22631. doi: 10.1371/journal.pone.0022631. Epub 2011 Aug 10.
A common technique used for sensitive and specific diagnostic virus detection in clinical samples is PCR that can identify one or several viruses in one assay. However, a diagnostic microarray containing probes for all human pathogens could replace hundreds of individual PCR-reactions and remove the need for a clear clinical hypothesis regarding a suspected pathogen. We have established such a diagnostic platform for random amplification and subsequent microarray identification of viral pathogens in clinical samples. We show that Phi29 polymerase-amplification of a diverse set of clinical samples generates enough viral material for successful identification by the Microbial Detection Array, demonstrating the potential of the microarray technique for broad-spectrum pathogen detection. We conclude that this method detects both DNA and RNA virus, present in the same sample, as well as differentiates between different virus subtypes. We propose this assay for diagnostic analysis of viruses in clinical samples.
聚合酶链式反应（PCR）是一种常用于临床样本中敏感且特异的病毒诊断检测的技术，它可以在一次检测中鉴定一种或几种病毒。然而，包含所有人类病原体探针的诊断微阵列可以替代数百种个体 PCR 反应，并消除对可疑病原体的明确临床假设的需求。我们已经建立了这样的诊断平台，用于随机扩增和随后的临床样本中病毒病原体的微阵列鉴定。我们表明，Phi29 聚合酶扩增的多样化临床样本可以产生足够的病毒物质，用于微生物检测阵列的成功鉴定，证明了微阵列技术在广谱病原体检测中的潜力。我们得出结论，该方法可以检测同一样本中的 DNA 和 RNA 病毒，并区分不同的病毒亚型。我们建议将该方法用于临床样本中病毒的诊断分析。
