Kokare Dadasaheb M, Shelkar Gajanan P, Borkar Chandrashekhar D, Nakhate Kartik T, Subhedar Nishikant K
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Amravati Road, Nagpur, Maharashtra, 440 033, India.
J Pharmacol Toxicol Methods. 2011 Nov-Dec;64(3):246-50. doi: 10.1016/j.vascn.2011.08.002. Epub 2011 Aug 11.
Stereotaxic administration of neuroactive agents, either in ventricles, or targeted at specific intracranial sites, is a widely employed strategy for neurological studies in rodents. Surgical implantation of cannula on the skull is particularly useful in chronic treatments. We describe a simple, inexpensive and reliable method to fabricate a cannula system for delivery of drugs at the targeted sites in the brain of rat or mouse.
The system consists of a guide cannula made from a hypodermic needle (24 gauge), a stainless steel wire (30 gauge) that serves as a dummy cannula, and an internal cannula made of stainless steel needle (30 gauge) taken from a hypodermic syringe. The cannula can be implanted by routine stereotaxic procedure and used for acute or chronic drug administration to conscious, free moving animals.
With a view to test the system for accuracy, the guide cannula was stereotaxically implanted, and neuropeptide Y was directly delivered into the lateral ventricle. These rats showed a significant increase in food intake. Another set of rats were cannulated for chronic protocol, wherein ethanol was delivered directly into the ventral tegmental area. In operant chamber, these rats showed increased ethanol self-administration. The proposed cannula takes around 5 min to fabricate and costs less than a dollar.
We feel that it may serve as an economical and reliable tool in neuropharmacological and neurobehavioral studies.
向脑室或特定颅内部位立体定向注射神经活性药物，是啮齿动物神经学研究中广泛采用的策略。在颅骨上手术植入套管在慢性治疗中特别有用。我们描述了一种简单、廉价且可靠的方法，用于制造一种套管系统，以便在大鼠或小鼠脑内的目标部位给药。
该系统由一根由皮下注射针（24号）制成的引导套管、一根用作假套管的不锈钢丝（30号）以及一根取自皮下注射器的不锈钢针（30号）制成的内套管组成。该套管可通过常规立体定向程序植入，并用于对清醒、自由活动的动物进行急性或慢性药物给药。
为了测试该系统的准确性，将引导套管立体定向植入，然后将神经肽Y直接注入侧脑室。这些大鼠的食物摄入量显著增加。另一组大鼠进行了慢性实验，将乙醇直接注入腹侧被盖区。在操作箱中，这些大鼠的乙醇自我给药量增加。所提出的套管制作大约需要5分钟，成本不到一美元。
我们认为它可作为神经药理学和神经行为学研究中一种经济且可靠的工具。
