Effect of neuraminidase treatment on persistent epileptiform activity in the rat hippocampus.

Negatively charged sialic acid residues located close to pores of voltage-gated sodium channels substantially influence their gating properties. The in vitro low Mg²⁺ seizure model is used to emulate difficult-to-treat status epilepticus. Using this model on cultured hippocampal slices, we examined the effectiveness of desialylation in reducing persistent seizure-like activity. We show that desialylation in cultured hippocampal slices effectively suppresses seizure-like activity induced by low Mg²⁺. These findings suggest that targeting negatively charged sialic acids may be an effective strategy to treat status epilepticus.