Vertox Laboratory, Defence Research and Development Establishment, Gwalior, India.
J Chromatogr A. 2011 Sep 23;1218(38):6612-20. doi: 10.1016/j.chroma.2011.07.091. Epub 2011 Aug 6.
Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX. Critical extraction parameters such as modifier concentration, nature and volume of washing and eluting solvents were investigated. Amongst other anionic compounds, selectivity in extraction was observed for organophosphorus compounds. Recoveries of analytes were determined by GC-MS which ranged from 80% to 115%. Comparison of zirconia based solid-phase extraction method with anion-exchange solid-phase extraction revealed its selectivity towards phosphonic acids. The limits of detection (LOD) and limit of quantification (LOQ) with selected analytes were achieved down to 4.3 and 8.5 ng mL(-1), respectively, in selected ion monitoring mode.
使用基于氧化锆的商业固相萃取小柱已实现对神经毒剂降解产物的选择性提取和富集。目标分析物为 O-烷基烷基膦酸和烷基膦酸，它们是沙林、梭曼和 VX 等神经毒剂的环境标志物。研究了关键的萃取参数，如改性剂浓度、洗涤和洗脱溶剂的性质和体积。在其他阴离子化合物中，观察到对有机磷化合物的萃取选择性。通过气相色谱-质谱法（GC-MS）测定了分析物的回收率，范围为 80%至 115%。与阴离子交换固相萃取相比，基于氧化锆的固相萃取方法对膦酸具有选择性。在选定的离子监测模式下，选定分析物的检测限（LOD）和定量限（LOQ）分别达到 4.3 和 8.5 ng mL(-1)。
