1,N6-乙烯腺嘌呤单磷酸与核苷酸相互作用的荧光研究。

Fluorescence studies of the interaction between 1,N6-ethenoadenosine monophosphate and nucleotides.

作者信息

Kubota Y, Motoda Y, Nakamura H

出版信息

Biophys Chem. 1979 Jan;9(2):105-9. doi: 10.1016/0301-4622(79)87004-0.

Abstract

AMP, GMP, TMP and CMP quench the fluorescence of 1,N6-ethenoadenosine monophosphate (epsilon-AMP). The fluorescence spectrum of epsilon-AMP-nucleotide system is identical with that of epsilon-AMP itself, and the fluorescence decay kinetics follow a single-exponential decay law. The dependence of fluorescence yields and lifetimes upon the concentration of nucleotides shows that the fluorescence of epsilon-AMP is principally quenched in a dynamic process by AMP, TMP and CMP, while it is quenched in both dynamic and static processes by GMP. The quenching constants increase in the following order: GMP greater than AMP greater than TMP greater than CMP.

摘要

腺苷一磷酸（AMP）、鸟苷一磷酸（GMP）、胸苷一磷酸（TMP）和胞苷一磷酸（CMP）可淬灭1,N6-乙烯腺苷单磷酸（ε-AMP）的荧光。ε-AMP-核苷酸体系的荧光光谱与ε-AMP本身的相同，且荧光衰减动力学遵循单指数衰减规律。荧光产率和寿命对核苷酸浓度的依赖性表明，ε-AMP的荧光主要在动态过程中被AMP、TMP和CMP淬灭，而在动态和静态过程中均被GMP淬灭。淬灭常数按以下顺序增加：GMP＞AMP＞TMP＞CMP。