McCurdy H D, Ho J, Dobson W J
Can J Microbiol. 1978 Dec;24(12):1475-81. doi: 10.1139/m78-237.
Exogenous cyclic nucleotide phosphodiesterase (PD) accelerated fruiting body (FB) formation and increased territory size of aggregates in Myxococcus xanthus. Both guanosine 3'5'-monophosphate (cGMP) and guanosine 5'-monophosphate (GMP) were antagonistic to the PD effect. Adenosine 3'5'-monophosphate (cAMP) increases FB numbers twofold in the absence but not in the presence of PD. PD induction is not affected by methionine or isoleucine, which inhibit, or by threonine, which stimulates, FB formation. There is an increase and subsequent decrease in cAMP levels during early glycerol-induced microcyst development but 10 mM theophylline or caffeine not only inhibited microcyst development but induced germination in the presence of glycerol. On the basis of these results and the reports of other investigators a tentative model is proposed based on a dual role for cyclic nucleotides in the development in M. xanthus.
外源性环核苷酸磷酸二酯酶（PD）加速了黄色粘球菌子实体（FB）的形成，并增加了聚集体的领地大小。鸟苷3',5'-单磷酸（cGMP）和鸟苷5'-单磷酸（GMP）均拮抗PD的作用。在不存在PD的情况下，腺苷3',5'-单磷酸（cAMP）可使FB数量增加两倍，但在存在PD时则不然。PD的诱导不受抑制FB形成的蛋氨酸或异亮氨酸的影响，也不受刺激FB形成的苏氨酸的影响。在甘油诱导的早期微囊发育过程中，cAMP水平先升高后降低，但10 mM的茶碱或咖啡因不仅抑制微囊发育，还在有甘油存在的情况下诱导萌发。基于这些结果以及其他研究者的报告，提出了一个基于环核苷酸在黄色粘球菌发育中的双重作用的初步模型。
