Ben-Ishay Z, Prindull G, Yankelev S, Sharon S
Laboratory of Experimental Hematology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Med Oncol Tumor Pharmacother. 1990;7(1):55-9. doi: 10.1007/BF03000491.
A study of treated murine acute myeloid leukemia (AML) with an emphasis on the bone marrow stromal function is reported. Leukemia was induced in C57Bl mice through intraperitoneal (i.p.) inoculation of C-1498 myelogenous leukemic cells. The leukemic mice were administered: (1) total body lethal X-irradiation (t.b.i.); (2) two i.p. cytosine-arabinoside (Ara-C) injections followed by X-irradiation. Control mice received similar regimens. Bone marrow of experimental and control mice was processed for stromal cell cultures (SCC) and in vitro engraftment of hematopoietic cells onto the cultures. The results of this study indicate that the bone marrow stromal deficiency which occurs in leukemia is aggravated by Ara-C and irradiation treatments. Moreover, SCC of treated leukemic mice sustain in vitro hematopoiesis only to a limited degree. Stromal deficiency, as possible cause for graft failure in bone marrow transplanted leukemic patients, is discussed.
本文报道了一项针对经治疗的小鼠急性髓系白血病（AML）的研究，重点关注骨髓基质功能。通过腹腔内（i.p.）接种C - 1498骨髓性白血病细胞，在C57Bl小鼠中诱导白血病。给白血病小鼠施用：（1）全身致死性X射线照射（t.b.i.）；（2）两次腹腔内注射阿糖胞苷（Ara - C），随后进行X射线照射。对照小鼠接受类似方案。对实验小鼠和对照小鼠的骨髓进行处理，用于基质细胞培养（SCC）以及造血细胞在体外接种到培养物上。本研究结果表明，白血病中出现的骨髓基质缺陷会因Ara - C和照射治疗而加重。此外，经治疗的白血病小鼠的SCC在体外仅能有限地维持造血。讨论了基质缺陷作为骨髓移植白血病患者移植失败的可能原因。
