Poirier Colline, Van der Linden Anne-Marie
Bio-Imaging Lab, University of Antwerp, 2020 Antwerp, Belgium.
Methods Mol Biol. 2011;771:569-76. doi: 10.1007/978-1-61779-219-9_29.
The advent of high-field MRI systems has allowed implementation of BOLD fMRI on small animals. Increased magnetic field improves the signal-to-noise ratio and thus allows improvement of spatial resolution. However, it also increases susceptibility artefacts in the commonly acquired gradient echo images. The problem is particularly challenging in songbirds due to the presence of numerous air cavities in the skull of birds. This problem can be solved by using spin echo BOLD fMRI. In this chapter, we describe how to use this technique in zebra finches, a small songbird of 15-25 g extensively studied in behavioural neurosciences of birdsong. The protocol implements auditory stimuli.
高场强MRI系统的出现使得在小动物身上实施BOLD功能磁共振成像（fMRI）成为可能。磁场强度的增加提高了信噪比，从而能够提高空间分辨率。然而，这也增加了在常见的梯度回波图像中出现的磁化率伪影。由于鸟类头骨中存在大量气腔，这个问题在鸣禽中尤为棘手。使用自旋回波BOLD fMRI可以解决这个问题。在本章中，我们将描述如何在斑胸草雀（一种体重为15 - 25克的小型鸣禽，在鸟类行为神经科学领域得到广泛研究）身上使用这项技术。该方案采用听觉刺激。
