[nm23-H1小干扰RNA增强肺腺癌细胞对脂质体包裹紫杉醇的体外化疗敏感性]

[nm23-H1-siRNA enhances the chemosensitivity to liposome-encapsulated paclitaxel in lung adenocarcinoma cells in vitro].

作者信息

Yang Xue-qin, Zhang Zhi-min, Wang Dong, Wang Ge, Zeng Ling-li, Yang Zhen-zhou

机构信息

Cancer Center, Daping Hospital and Research Institute of Surgery, The Third Military Medical University, Chongqing 400042, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2011 Jun;33(6):405-9.

PMID:21875478

Abstract

OBJECTIVE

To study the chemosensitivity of lung adenocarcinoma cell line A549 cells to liposome-encapsulated paclitaxel after treatment by nm23-H1-small interference RNA (nm23-H1-siRNA) in vitro.

METHODS

The A549 cells were divided into two groups: non-transfected group and nm23-H1-siRNA-transfected group. Western blot analysis was used to detect the expression of nm23-H1. MTT and flow cytometry were used to determine the cell mortality rate, apoptosis rate and cell cycle after liposome-encapsulated paclitaxel treatment in both groups.

RESULTS

The expression of nm23-H1 in A549 cells was significantly decreased after transfection with nm23-H1-siRNA. After treatment for 48 hours with liposome-encapsulated paclitaxel, the cell mortality rate was increased with the increasing concentration of liposome-encapsulated paclitaxel in both groups, but increased higher in the nm23-H1-siRNA-transfected group. When the concentration of liposome-encapsulated paclitaxel was above 5 µg/ml, the cell mortality rate was significantly higher than that in the non-transfected group (P < 0.05). The proportion of apoptotic cells also increased in the nm23-H1-siRNA-transfected group, compared with that of the non-transfected group (t = 3.812, P < 0.05), while the proportion of cells at S and G(2)/M phase decreased after transfection with nm23-H1-siRNA (S phase:t = 8.356, P < 0.05; G(2)/M phase:t = 7.256, P < 0.05).

CONCLUSIONS

Nm23-H1 is related with the chemoresistance to liposome-encapsulated paclitaxel in lung adenocarcinoma cell line A549 cells. Inhibition of the expression of nm23-H1 by nm23-H1-siRNA can improve the in vitro chemosensitivity of A549 cells to liposome-encapsulated paclitaxel.

摘要

目的

研究体外经nm23-H1小干扰RNA（nm23-H1-siRNA）处理后肺腺癌细胞系A549细胞对脂质体包裹紫杉醇的化学敏感性。

方法

将A549细胞分为两组：未转染组和nm23-H1-siRNA转染组。采用蛋白质免疫印迹分析检测nm23-H1的表达。采用MTT法和流式细胞术测定两组细胞经脂质体包裹紫杉醇处理后的细胞死亡率、凋亡率及细胞周期。

结果

用nm23-H1-siRNA转染后，A549细胞中nm23-H1的表达明显降低。脂质体包裹紫杉醇处理48小时后，两组细胞死亡率均随脂质体包裹紫杉醇浓度的增加而升高，但nm23-H1-siRNA转染组升高更明显。当脂质体包裹紫杉醇浓度高于5μg/ml时，细胞死亡率明显高于未转染组（P<0.05）。与未转染组相比，nm23-H1-siRNA转染组凋亡细胞比例也增加（t=3.812，P<0.05），而用nm23-H1-siRNA转染后S期和G(2)/M期细胞比例下降（S期：t=8.356，P<0.05；G(2)/M期：t=7.256，P<0.05）。