Department of Bioorganic Chemistry, Radboud University Nijmegen, Heyendaalseweg 135, NL-6525 AJ Nijmegen, The Netherlands.
Angew Chem Int Ed Engl. 2011 Sep 12;50(38):8806-27. doi: 10.1002/anie.201008102. Epub 2011 Sep 2.
In 1919 the German chemist Hermann Staudinger was the first to describe the reaction between an azide and a phosphine. It was not until recently, however, that Bertozzi and co-workers recognized the potential of this reaction as a method for bioconjugation and transformed it into the so-called Staudinger ligation. The bio-orthogonal character of both the azide and the phosphine functions has resulted in the Staudinger ligation finding numerous applications in various complex biological systems. For example, the Staudinger ligation has been utilized to label glycans, lipids, DNA, and proteins. Moreover, the Staudinger ligation has been used as a synthetic method to construct glycopeptides, microarrays, and functional biopolymers. In the emerging field of bio-orthogonal ligation strategies, the Staudinger ligation has set a high standard to which most of the new techniques are often compared. This Review summarizes recent developments and new applications of the Staudinger ligation.
1919 年，德国化学家赫尔曼·施陶丁格（Hermann Staudinger）首次描述了叠氮化物与膦之间的反应。然而，直到最近，贝托齐（Bertozzi）及其同事才认识到该反应作为生物偶联方法的潜力，并将其转化为所谓的施陶丁格连接反应。叠氮化物和膦官能团的生物正交特性使得施陶丁格连接反应在各种复杂的生物系统中找到了众多应用。例如，施陶丁格连接反应已被用于标记聚糖、脂质、DNA 和蛋白质。此外，施陶丁格连接反应已被用作构建糖肽、微阵列和功能性生物聚合物的合成方法。在新兴的生物正交连接策略领域，施陶丁格连接反应设定了一个高标准，大多数新技术通常都与之进行比较。这篇综述总结了施陶丁格连接反应的最新发展和新应用。
