Cilla A, Laparra J M, Alegria A, Barbera R
Department of Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Burjassot, Valencia, Spain.
Nutr Hosp. 2011 May-Jun;26(3):614-21. doi: 10.1590/S0212-16112011000300027.
Fruit beverages are commonly supplemented with milk, vitamins and/or minerals in order to improve their healthy effects by providing some bioactive components that can act additively or synergistically against oxidative stress.
To test whether iron, zinc, and milk added to fruit beverages do not affect the cytoprotective effect against oxidative damage to Caco-2 cells through GSH-related enzymes induction and cell cycle progression preservation, in comparison with non-supplemented fruit beverage.
Caco-2 cells were incubated 24 h with the bioaccessible fraction (BF) of eight fruit beverages with/without iron and/or zinc, and/or milk, and then challenged with H2O2 (5 mmol L-1 -2 h). Mitochondrial enzyme activities (MTT test), GSH-Rd and GSH-Px enzyme activities, cell cycle progression and caspase-3 activity were measured.
Fruit beverages prevented the deleterious effect of H2O2 on cell viability, with almost all samples reaching control basal levels. Only independent iron or zinc supplementation with/without milk exerted positive effects upon GSH-Rd activity. Both minerals with milk, afforded improved preservation of GSH-Px activity. All samples prevented the decrease in the G1 phase of cell cycle induced by H2O2, except iron supplemented samples with/without milk, but none of them avoided the increase in sub-G1 phase. However, this fact was not associated to caspase-3 activity, with a probable positive effect of zinc upon this parameter.
Mineral and/or milk supplementation of fruit beverages helps in the prevention of oxidative stress in Caco-2 cells based on cell viability maintenance, GSH-related enzymes activation, cell cycle distribution preservation and inhibition of caspase-3 activation.
水果饮料通常添加牛奶、维生素和/或矿物质，以通过提供一些可协同或相加作用对抗氧化应激的生物活性成分来增强其健康功效。
与未添加成分的水果饮料相比，测试添加到水果饮料中的铁、锌和牛奶是否不会通过诱导谷胱甘肽相关酶和维持细胞周期进程来影响对Caco-2细胞氧化损伤的细胞保护作用。
将Caco-2细胞与八种添加或未添加铁和/或锌以及/或者牛奶的水果饮料的生物可利用部分（BF）一起孵育24小时，然后用H2O2（5 mmol L-1 - 2小时）进行刺激。测量线粒体酶活性（MTT试验）、谷胱甘肽还原酶（GSH-Rd）和谷胱甘肽过氧化物酶（GSH-Px）活性、细胞周期进程和半胱天冬酶-3活性。
水果饮料可预防H2O2对细胞活力的有害影响，几乎所有样品均达到对照基础水平。仅单独添加铁或锌（无论是否添加牛奶）对GSH-Rd活性有积极影响。同时添加这两种矿物质和牛奶可更好地保持GSH-Px活性。除添加铁的样品（无论是否添加牛奶）外，所有样品均能预防H2O2诱导的细胞周期G1期减少，但均未避免亚G1期增加。然而，这一事实与半胱天冬酶-3活性无关，锌对此参数可能有积极影响。
水果饮料中添加矿物质和/或牛奶有助于预防Caco-2细胞中的氧化应激，其作用基于维持细胞活力、激活谷胱甘肽相关酶、保持细胞周期分布以及抑制半胱天冬酶-3激活。
