Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan.
J Sep Sci. 2011 Oct;34(20):2906-12. doi: 10.1002/jssc.201100429. Epub 2011 Aug 24.
A fluorescence detection system for a microfluidic device using an organic light-emitting diode (OLED) as the excitation light source and a charge-coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm(2) was 13 μW (1.7 mW/cm(2)). The fluorescence detection system consisted of the OLED device, two band-pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow-based enzyme-linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96-well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.
开发了一种使用有机发光二极管 (OLED) 作为激发光源和电荷耦合器件 (CCD) 作为光电探测器的微流控装置荧光检测系统。通过光刻和真空沉积技术在玻璃基板上制造 OLED。OLED 产生峰值发射为 512nm、半带宽为 55nm 的绿光。OLED 的最大外量子效率为 7.2%。在 10mA/cm(2) 时，OLED 的发射强度为 13μW（1.7mW/cm(2)）。荧光检测系统由 OLED 器件、两个带通滤波器、一个五微通道聚二甲基硅氧烷 (PDMS) 微流控装置和一个线性 CCD 组成。该荧光检测系统成功地用于 PDMS 微流控装置上的基于流动的酶联免疫吸附测定，用于快速测定免疫球蛋白 A (IgA)，这是人类压力的标志物。IgA 的检测限 (S/N=3) 为 16.5ng/mL，灵敏度足以评估压力。与传统的 96 孔微量滴定板测定法相比，分析时间以及试剂和样品溶液的用量都可以减少。
