Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA 02543, USA.
ISME J. 2012 Feb;6(2):309-19. doi: 10.1038/ismej.2011.108. Epub 2011 Sep 8.
The Moorea Coral Reef Long Term Ecological Research (LTER) Site (17.50°S, 149.83°W) comprises the fringe of coral reefs and lagoons surrounding the volcanic island of Moorea in the Society Islands of French Polynesia. As part of our Microbial Inventory Research Across Diverse Aquatic LTERS biodiversity inventory project, we characterized microbial community composition across all three domains of life using amplicon pyrosequencing of the V6 (bacterial and archaeal) and V9 (eukaryotic) hypervariable regions of small-subunit ribosomal RNA genes. Our survey spanned eight locations along a 130-km transect from the reef lagoon to the open ocean to examine changes in communities along inshore to offshore gradients. Our results illustrate consistent community differentiation between inshore and offshore ecosystems across all three domains, with greater richness in all domains in the reef-associated habitats. Bacterial communities were more homogenous among open ocean sites spanning >100 km than among inshore sites separated by <1 km, whereas eukaryotic communities varied more offshore than inshore, and archaea showed more equal levels of dissimilarity among subhabitats. We identified signature communities representative of specific geographic and geochemical milieu, and characterized co-occurrence patterns of specific microbial taxa within the inshore ecosystem including several bacterial groups that persist in geographical niches across time. Bacterial and archaeal communities were dominated by few abundant taxa but spatial patterning was consistent through time and space in both rare and abundant communities. This is the first in-depth inventory analysis of biogeographic variation of all three microbial domains within a coral reef ecosystem.
莫雷阿珊瑚礁长期生态研究（LTER）站（17.50°S，149.83°W）位于法属波利尼西亚社会群岛的莫雷阿火山岛周围的珊瑚礁和泻湖边缘。作为我们跨多样水生 LTER 生物多样性调查项目的微生物组学调查部分，我们使用 V6（细菌和古菌）和 V9（真核生物）小亚基核糖体 RNA 基因高变区的扩增子焦磷酸测序，对所有三个生命领域的微生物群落组成进行了特征描述。我们的调查跨越了从泻湖到开阔海域的 130 公里长的 8 个地点，以检查沿岸到近海梯度上群落的变化。我们的研究结果表明，所有三个领域的近岸和近海生态系统之间存在一致的群落分化，与近岸生境相关的所有领域的丰富度都更高。在跨越 100 多公里的开阔海域站点之间，细菌群落比在相距不到 1 公里的近岸站点之间更加同质，而真核生物群落的变化比近岸更大，古菌在亚生境之间的差异水平也更加均等。我们确定了具有特定地理和地球化学环境代表性的特征群落，并描述了近岸生态系统中特定微生物类群的共现模式，包括在时间和地理范围内在地理小生境中持续存在的几个细菌群体。细菌和古菌群落主要由少数丰富的类群主导，但稀有和丰富群落的时空分布模式一致。这是对珊瑚礁生态系统中所有三个微生物领域的生物地理变化进行的首次深入清单分析。
