Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA.
Bioresour Technol. 2011 Nov;102(21):10028-34. doi: 10.1016/j.biortech.2011.08.057. Epub 2011 Aug 19.
A simple pretreatment method using anhydrous ammonia was developed to minimize water and ammonia inputs for cellulosic ethanol production, termed the low moisture anhydrous ammonia (LMAA) pretreatment. In this method, corn stover with 30-70% moisture was contacted with anhydrous ammonia in a reactor under nearly ambient conditions. After the ammoniation step, biomass was subjected to a simple pretreatment step at moderate temperatures (40-120°C) for 48-144 h. Pretreated biomass was saccharified and fermented without an additional washing step. With 3% glucan loading of LMAA-treated corn stover under best treatment conditions (0.1g-ammonia+1.0 g-water per g biomass, 80°C, and 84 h), simultaneous saccharification and cofermentation test resulted in 24.9 g/l (89% of theoretical ethanol yield based on glucan+xylan in corn stover).
开发了一种使用无水氨的简单预处理方法，以最小化纤维素乙醇生产的水和氨的投入，称为低水分无水氨（LMAA）预处理。在该方法中，将水分含量为 30-70%的玉米秸秆在接近环境条件的反应器中与无水氨接触。氨化步骤后，生物质在中等温度（40-120°C）下进行简单预处理步骤 48-144 小时。预处理后的生物质无需额外的洗涤步骤即可进行糖化和发酵。在最佳处理条件下（每克生物质用 0.1g-氨+1.0g-水，80°C，84 小时），用 3%的纤维素负载量的 LMAA 处理的玉米秸秆进行同步糖化和共发酵试验，得到 24.9g/L（基于玉米秸秆中纤维素+xylan 的理论乙醇产率的 89%）。
