Department of Genetics of Microorganisms, University of Łódź, Poland.
Pol J Microbiol. 2011;60(2):145-8.
Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T rubrum (CBS 120358), T mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.
基于 PCR 指纹图谱的皮肤癣菌（dermatophytes）分子分析显示属和种之间存在高度的克隆分化。犬小孢子菌（zoophilic dermatophyte，属于 Microsporum 属）是引起儿童头癣、成人体癣和猫癣的主要病原体，与其他皮肤癣菌相比非常独特。大多数分子研究的结果表明，犬小孢子菌内部没有克隆分化，与其他物种不同。本研究旨在应用（GACA）4 重复引物和（ACA）5 引物对来自波兰中部的人和动物的犬小孢子菌菌株进行分型。真菌菌株：32 株来自波兰中部患者的临床分离犬小孢子菌；11 株来自感染猫（6 只）和狗（7 只）的菌株，参考株犬小孢子菌（CBS 113480）、红色毛癣菌（T rubrum，CBS 120358）、须癣毛癣菌（T mentagrophytes，CBS 120357）和絮状表皮癣菌（E. floccosum，CBS 970.95）。用 RAPD 反应以分析的犬小孢子菌菌株的基因组 DNA 作为模板。使用（GACA）4 和（ACA）5 分型未观察到分析的犬小孢子菌菌株的分化。
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