Xiao Yue-hai, Sun Fa, Li Chong-bin, Shi Jia-qi, Gu Jiang, Xie Chun, Guan Zhi-zhong, Yu Yan-ni
Department of Urology, Affiliated Hospital of Guiyang Medical College, Guiyang, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2011 Aug;33(4):357-61. doi: 10.3881/j.issn.1000-503X.2011.04.002.
To explore the effect of endemic fluoride poisoning caused by coal burning on the oxidative stress in rat testis.
Totally 40 male SD rats were equally randomized into four groups control group, low fluorosis group, middle fluorosis group, and high fluorosis group. Rats in all three fluorosis groups were fed with corn dried by burning coal obtained from endemic fluorosis areas with high fluoride, and thus the animal models of fluorosis were established. After 120 and 180 days, all the rats were sacrificed. Testis tissues were stained with hematoxylin eosin and observed under light microscope. The malonaldehyde (MDA) content, superoxide dismutase (SOD) activity, total nitric oxide synthase (TNOS), and inducible nitric oxidase synthase (iNOS) were measured by biochemical methods in the testis tissues. The content of NaF in testis was measured by fluorine selective electrode.
The rat fluorosis models were successfully established. The fluoride content in testis was significantly increased in all the fluorosis groups(P<0.01). Testicular structures were damaged in all of fluoride groups. The TNOS, iNOS activities, and MDA content of each fluoride group were significantly higher than that of the control group on day 120 and 180 (P<0.05 or 0.01 ). The TNOS, iNOS activities, and MDA content significantly increased in a dose dependent manner (P<0.05 or 0.01). The SOD activities significantly decreased in all the fluoride groups (P<0.05 or 0.01).
Endemic fluoride poisoning caused by coal burning can cause disorders in the oxidative system and antioxidative system in rat testis. The oxidative stress may play an important role in the fluorides induced reproductive toxicity in male rats.
探讨燃煤型地方性氟中毒对大鼠睾丸氧化应激的影响。
将40只雄性SD大鼠随机分为4组，即对照组、轻度氟中毒组、中度氟中毒组和重度氟中毒组。3个氟中毒组大鼠均喂饲采自高氟地方性氟中毒病区的燃煤烘干玉米，从而建立氟中毒动物模型。120天和180天后，处死所有大鼠。睾丸组织用苏木精-伊红染色，在光学显微镜下观察。采用生化方法检测睾丸组织中丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性、总一氧化氮合酶（TNOS）和诱导型一氧化氮合酶（iNOS）。用氟离子选择性电极测定睾丸中氟化钠含量。
成功建立大鼠氟中毒模型。各氟中毒组睾丸中氟含量显著升高（P<0.01）。所有氟中毒组睾丸结构均受损。各氟中毒组在120天和180天时TNOS、iNOS活性及MDA含量均显著高于对照组（P<0.05或0.01）。TNOS、iNOS活性及MDA含量呈剂量依赖性显著升高（P<0.05或0.01）。所有氟中毒组SOD活性均显著降低（P<0.05或0.01）。
燃煤型地方性氟中毒可导致大鼠睾丸氧化系统和抗氧化系统紊乱。氧化应激可能在氟诱导的雄性大鼠生殖毒性中起重要作用。
