Department of Chemistry, University of Minnesota, 207 Pleasant St. S.E., Minneapolis, MN 55455, USA.
J Chromatogr A. 2012 Mar 9;1228:110-24. doi: 10.1016/j.chroma.2011.07.096. Epub 2011 Aug 6.
A new family of hyper-crosslinked (HC) phases for use under very aggressive acid conditions including those encountered in ultra-fast, high temperature two-dimensional liquid chromatography (2DLC) has been recently introduced. This type of stationary phase shows significantly enhanced acid and thermal stability compared to the most acid stable, commercial RPLC phases. In addition, the use of "orthogonal" chemistry to make surface-confined polymer networks ensures good reproducibility and high efficiency. One of the most interesting features of the HC phases is the ability to derivatize the surface aromatic groups with various functional groups. This has led to the development of a family of hyper-crosslinked phases possessing a wide variety of chromatographic selectivities by attaching hydrophobic (e.g. -C₈), ionizable (e.g. -COOH, -SO₃H), aromatic (e.g. -toluene) or polar (e.g. -OH) species to the aromatic polymer network. HC reversed phases with various degrees of hydrophobicity and mixed-mode HC phases with added strong and weak cation exchange sites have been synthesized, characterized and applied. These silica-based acid-stable HC phases, with their attractive chromatographic properties, should be very useful in the separation of bases or biological analytes in acidic media, especially at elevated temperatures. This work reviews prior research on HC phases and introduces a novel HC phase made by alternative chemistry.
最近引入了一种新的超交联 (HC) 相家族，可在非常苛刻的酸性条件下使用，包括在超快速、高温二维液相色谱 (2DLC) 中遇到的条件。与最耐酸的商业反相高效液相色谱 (RPLC) 相相比，这种固定相具有显著增强的酸稳定性和热稳定性。此外，使用“正交”化学方法制造表面受限的聚合物网络可确保良好的重现性和高效率。HC 相最有趣的特点之一是能够用各种官能团对表面芳族基团进行衍生化。这导致了一系列具有广泛色谱选择性的超交联相的发展，通过将疏水性（例如-C₈）、可离子化（例如-COOH、-SO₃H）、芳族（例如-甲苯）或极性（例如-OH）物种附着到芳族聚合物网络上。已经合成、表征和应用了具有不同疏水性程度的 HC 反相相和具有添加的强和弱阳离子交换位点的混合模式 HC 相。这些基于硅胶的耐酸 HC 相具有吸引人的色谱性质，在酸性介质中分离碱基或生物分析物（尤其是在高温下）时应该非常有用。这项工作回顾了 HC 相的先前研究，并介绍了一种通过替代化学方法制造的新型 HC 相。
