利用氢化酶和乙醇脱氢酶组合通过氢气实现NADH再生及酮氢化。科学笔记。
Regeneration of NADH and ketone hydrogenation by hydrogen with the combination of hydrogenase and alcohol dehydrogenase. Scientific note.
作者信息
Okura I, Otsuka K, Nakada N, Hasumi F
机构信息
Department of Bioengineering, Tokyo Institute of Technology, Japan.
出版信息
Appl Biochem Biotechnol. 1990 Spring-Summer;24-25:425-30. doi: 10.1007/BF02920266.
PMID:2191625
Abstract
The regeneration of nicotinamide-adenine dinucleotide (reduced form, NADH) by the reaction of NAD with hydrogen gas was carried out in the presence of the hydrogenase from Alcaligenes eutrophus. And the formations of alcohol, CO2, and 6-phospho-gluconate were observed by a combination of the above system and corresponding dehydrogenases. NADH was regenerated by hydrogen gas with the hydrogenase and recycled in these reactions.
摘要
在嗜糖产碱菌氢化酶存在的情况下，通过烟酰胺腺嘌呤二核苷酸（还原型，NADH）与氢气的反应实现了NADH的再生。通过上述系统与相应脱氢酶的组合，观察到了乙醇、二氧化碳和6-磷酸葡萄糖酸的生成。NADH通过氢化酶利用氢气进行再生，并在这些反应中循环利用。
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