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IL-18 在非妊娠、妊娠和流产大鼠下丘脑-垂体-卵巢轴中的表达和定位。

Expression and localization of IL-18 in the hypothalamic-pituitary-ovarian axis of non-pregnant, pregnant, and abortive rats.

机构信息

Institute of Stem cell and tissue engineering, Binzhou Medical University, Yantai, Shandong 264003, China.

出版信息

J Reprod Immunol. 2011 Dec;92(1-2):45-53. doi: 10.1016/j.jri.2011.07.002. Epub 2011 Sep 13.

Abstract

Cytokines present in the reproductive system play an important role both in the modulation of immune responses to infectious challenge and in the establishment and maintenance of pregnancy. Interleukin 18 (IL-18) has been regarded as an important regulator of innate and acquired immune response, but its expression and distribution in the hypothalamic-pituitary-ovarian axis remain unclear. In this paper, the expression and distribution of IL-18 in non-pregnant, pregnant, and early abortive rats were examined using an ultra-sensitive immunohistochemical streptavidin-peroxidase method, enzyme-linked immunosorbent assay, and reverse transcription-polymerase chain reaction. The results showed that IL-18 expression in the pituitary, in follicular ovaries, and in the corpus luteum of abortive rats were significantly lower than that of pregnant and non-pregnant rats. However, the staining of IL-18 in the hypothalamus, interstitial glands of the ovary, and uterus of abortive rats was strikingly stronger than those of the non-pregnant ones. IL-18 mRNA expression in rat uterus was detected in all groups, whereas IL-18 mRNA content in abortive rat uterus was significantly higher than in normal pregnant rats. Further, IL-18 in the peripheral blood serum of abortive rats was significantly lower than in same-period normal pregnant rats. The differential expression of IL-18 in early abortion suggests that IL-18 may be related to the underlying mechanisms of abortion.

摘要

生殖系统中存在的细胞因子在调节对感染性挑战的免疫反应以及建立和维持妊娠方面发挥着重要作用。白细胞介素 18(IL-18)一直被认为是先天和获得性免疫反应的重要调节剂,但它在下丘脑-垂体-卵巢轴中的表达和分布尚不清楚。本文采用超敏免疫组织化学链霉亲和素-过氧化物酶法、酶联免疫吸附试验和逆转录-聚合酶链反应检测了非妊娠、妊娠和早期流产大鼠中 IL-18 的表达和分布。结果表明,流产大鼠垂体、卵泡卵巢和黄体中 IL-18 的表达明显低于妊娠和非妊娠大鼠。然而,流产大鼠下丘脑、卵巢间质腺和子宫中 IL-18 的染色明显强于非妊娠大鼠。在所有组中均检测到大鼠子宫中 IL-18 的 mRNA 表达,而流产大鼠子宫中 IL-18 的 mRNA 含量明显高于正常妊娠大鼠。此外,流产大鼠外周血清中的 IL-18 明显低于同期正常妊娠大鼠。IL-18 在早期流产中的差异表达表明,IL-18 可能与流产的潜在机制有关。

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