State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
Plant Signal Behav. 2011 Oct;6(10):1578-80. doi: 10.4161/psb.6.10.17095. Epub 2011 Oct 1.
The dynamic remodeling of actin filaments in guard cells functions in stomatal movement regulation. In our previous study, we found that the stochastic dynamics of guard cell actin filaments play a role in chloroplast movement during stomatal movement. In our present study, we further find that tubular actin filaments are present in tobacco guard cells that express GFP-mouse talin; approximately 2.3 tubular structures per cell with a diameter and height in the range of 1-3 µm and 3-5 µm, respectively. Most of the tubular structures were found to be localized in the cytoplasm near the inner walls of the guard cells. Moreover, the tubular actin filaments altered their localization slowly in the guard cells of static stoma, but showed obvious remodeling, such as breakdown and re-formation, in moving guard cells. Tubular actin filaments were further found to be colocalized with the chloroplasts in guard cells, but their roles in stomatal movement regulation requires further investigation.
保卫细胞中肌动蛋白丝的动态重塑在气孔运动调节中起作用。在我们之前的研究中，我们发现保卫细胞肌动蛋白丝的随机动力学在气孔运动过程中叶绿体的运动中起作用。在本研究中，我们进一步发现，在表达 GFP-小鼠塔林的烟草保卫细胞中存在管状肌动蛋白丝；每个细胞大约有 2.3 个管状结构，直径和高度分别在 1-3μm 和 3-5μm 范围内。大多数管状结构被发现位于靠近保卫细胞内壁的细胞质中。此外，在静态气孔的保卫细胞中，管状肌动蛋白丝的定位缓慢变化，但在运动的保卫细胞中，它们表现出明显的重塑，如解体和重新形成。进一步发现管状肌动蛋白丝与保卫细胞中的叶绿体共定位，但它们在气孔运动调节中的作用还需要进一步研究。
