State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; Graduate School, Chinese Academy of Sciences, Beijing, China.
Dev Dyn. 2011 Oct;240(10):2281-9. doi: 10.1002/dvdy.22725. Epub 2011 Aug 26.
The bipolar spindle is a complex molecular machinery that drives chromosome congression and segregation. During meiosis in the mouse multiple microtubule organizing centers aggregate to form a bipolar intermediate followed by elongation and establishment of the barrel-shaped acentriolar meiotic spindle. Previous studies have shown that septin1 is localized to spindle poles in mitosis, suggesting its possible involvement in spindle assembly. We, therefore, asked whether perturbation of septin1 will impair the process of spindle assembly and investigated localization and function during mouse oocyte meiotic maturation. Septin1 was localized to the spindle at metaphase and at the midbody during cytokinesis. Disruption of septin1 function using siRNA caused a decrease in PBE and extensive spindle defects. Moreover, the process of chromosome congression was impaired. However, septin1 depletion did not cause aneuploidy in oocyte with an extruded polar body. Taken together, our results show that septin1 is a key player in spindle assembly and chromosome congression in mouse meiosis.
双极纺锤体是一种复杂的分子机器，可驱动染色体向两极的集中和分离。在小鼠减数分裂过程中，多个微管组织中心聚集形成双极中期纺锤体，随后纺锤体拉长并形成无中心体的桶状纺锤体。先前的研究表明， septin1 在有丝分裂中定位于纺锤体两极，提示其可能参与纺锤体的组装。因此，我们想知道 septin1 的扰动是否会影响纺锤体的组装过程，并研究其在小鼠卵母细胞减数分裂成熟过程中的定位和功能。septin1 在有丝分裂中期和胞质分裂的中期体上定位于纺锤体。使用 siRNA 破坏 septin1 功能会导致 PBE 减少和广泛的纺锤体缺陷。此外，染色体向两极的集中过程也受到了损害。然而，在挤出极体的卵母细胞中， septin1 的耗竭并没有导致非整倍体。综上所述，我们的结果表明， septin1 是小鼠减数分裂中纺锤体组装和染色体向两极集中的关键因素。
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