McLaughlin R W, Zheng J S, Chen M M, Zhao Q Z, Wang D
Biology Department, Saint Mary's University of Minnesota, Winona, Minnesota 55987-1399, USA.
Dis Aquat Organ. 2011 Jul 12;95(3):241-5. doi: 10.3354/dao02364.
In animals, infection by the Epsilonproteobacteria Helicobacter spp. and H. cetorum is widespread. It has been suggested that H. cetorum may cause gastritis in cetaceans. The aim of our study was to investigate the presence of Helicobacter spp. in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. The fecal material of 12 porpoises living in the wild in Poyang Lake and 1 porpoise living in captivity at the Wuhan Baiji Dolphinarium were examined by PCR for the presence of Helicobacter spp. The fecal material of 8 of 12 wild porpoises and the captive porpoise were positive for Helicobacter spp. as determined by PCR using Helicobacter-specific primers, which target the 16S rRNA gene. A 16S rRNA clone library was then prepared from 1 sample isolated from a female porpoise living in the wild. DNA sequence analysis from 3 of the clones showed 98 to 99% identity to the H. cetorum 16S rRNA gene. These results demonstrate the prevalence of Helicobacter spp. and H. cetorum in endangered freshwater finless porpoises.
在动物中，埃普西隆变形菌属的幽门螺杆菌（Helicobacter spp.）和鲸幽门螺杆菌（H. cetorum）感染十分普遍。有人认为鲸幽门螺杆菌可能会导致鲸类动物患胃炎。我们研究的目的是调查濒危物种长江江豚（Neophocaena phocaenoides asiaeorientalis）粪便中幽门螺杆菌的存在情况。通过聚合酶链反应（PCR）检测了12头生活在鄱阳湖的野生江豚以及1头饲养在武汉白鱀豚馆的圈养江豚的粪便，以确定其中是否存在幽门螺杆菌。使用针对16S rRNA基因的幽门螺杆菌特异性引物进行PCR检测，结果显示12头野生江豚中有8头以及那头圈养江豚的粪便中幽门螺杆菌呈阳性。随后从1头野生雌性江豚的样本中制备了16S rRNA克隆文库。对其中3个克隆进行的DNA序列分析表明，它们与鲸幽门螺杆菌16S rRNA基因的同一性为98%至99%。这些结果证明了幽门螺杆菌和鲸幽门螺杆菌在濒危淡水江豚中的流行情况。
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