Institute of Bioscience, Putra University, Malaysia, Serdang, 43400 Selangor, Malaysia.
Evid Based Complement Alternat Med. 2012;2012:786487. doi: 10.1155/2012/786487. Epub 2011 Sep 15.
The in vivo immunomodulatory effect of ethanolic extracts from leaves of Rhaphidophora korthalsii was determined via immune cell proliferation, T/NK cell phenotyping, and splenocyte cytotoxicity of BALB/c mice after 5 consecutive days of i.p. administration at various concentrations. Splenocyte proliferation index, cytotoxicity, peripheral blood T/NK cell population, and plasma cytokine (IL-2 and IFN-γ) in mice were assessed on day 5 and day 15. High concentration of extract (350 μg/mice/day for 5 consecutive days) was able to stimulate immune cell proliferation, peripheral blood NK cell population, IL-2, and IFN- γ cytokines, as well as splenocyte cytotoxicity against Yac-1 cell line. Unlike rIL-2 which degraded rapidly, the stimulatory effect from the extract managed to last until day 15. These results suggested the potential of this extract as an alternative immunostimulator, and they encourage further study on guided fractionation and purification to identify the active ingredients that contribute to this in vitro and in vivo immunomodulatory activity.
通过腹腔注射不同浓度的乙醇提取物，连续 5 天，检测 Rhaphidophora korthalsii 叶的体内免疫调节作用，观察 BALB/c 小鼠免疫细胞增殖、T/NK 细胞表型和脾细胞细胞毒性的变化。第 5 天和第 15 天检测小鼠脾细胞增殖指数、细胞毒性、外周血 T/NK 细胞群和血浆细胞因子（IL-2 和 IFN-γ）。高浓度的提取物（连续 5 天每天 350μg/只）能够刺激免疫细胞增殖、外周血 NK 细胞群、IL-2 和 IFN-γ细胞因子，以及脾细胞对 Yac-1 细胞系的细胞毒性。与迅速降解的 rIL-2 不同，提取物的刺激作用持续到第 15 天。这些结果表明该提取物具有作为免疫刺激剂的潜力，并鼓励进一步进行导向分离和纯化研究，以鉴定出对这种体外和体内免疫调节活性有贡献的活性成分。
