Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt.
Oper Dent. 2012 Jan-Feb;37(1):45-53. doi: 10.2341/11-166-L. Epub 2011 Sep 26.
This study investigated the effect of water storage on nanoleakage depth and the pattern of cervical cavities bonded with different adhesives.
For nanoleakage depth evaluation, standardized cervical cavities (2 mm in diameter) were prepared on the buccal and lingual surfaces of 36 intact human premolars. Specimens were divided into three groups (n=12) according to the three adhesive systems used: an etch-and-rinse adhesive (SBMP, Adper Scotchbond Multi-Purpose, 3M ESPE) and two single-step self-etch adhesives; one was mild and acetone based (IB-iBond, Kulzer), while the other was strong water based (PL, Adper Prompt L-Pop, 3M ESPE). All cavities were restored using Filtek Z250 (3M ESPE) resin composite. For each adhesive, specimens (n=12 with 24 restored cavities) were subdivided into three subgroups (n=4 with eight cavities) according to the storage period before examination (24 hours, three or six months). Another duplicate of teeth was prepared in the same way for nanoleakage pattern evaluation. After storage, the specimens were placed in 50%W/V silver nitrate solution for 24 hours and immersed in a photo-developing solution for eight hours. Thereafter, the specimens were sectioned buccolingually, polished, and examined by scanning electron microscopy. For nanoleakage pattern, specimens were treated in the same way as for nanoleakage depth except that they were additionally immersed in 10% EDTA for five seconds after polishing. Silver penetration percentage was calculated to the total length of the tooth-restoration interface. Data were analyzed with two-way analysis of variance, Kruskal-Wallis, and post hoc tests.
After 24 hours, the least amount of nanoleakage depth was recorded for IB, while the highest was recorded for PL. For stored specimens, there was no significant difference among the nanoleakage depths of all adhesives. The tested adhesives recorded different nanoleakage patterns; however, there was an increase in the intensity and continuity of silver deposition by time.
After 24 hours, the nanoleakage depth/pattern varied with the type of adhesive used; however, after water storage, all adhesives performed equally.
本研究旨在探讨不同粘接剂在储存水后对纳米渗漏深度和颈部腔模式的影响。
为了评估纳米渗漏深度，在 36 颗完整的人前磨牙的颊面和舌面制备了直径为 2mm 的标准化颈腔。根据使用的三种粘接系统（一种是酸蚀冲洗型粘接剂 SBMP，Adper Scotchbond Multi-Purpose，3M ESPE；两种是单步自酸蚀粘接剂，一种是温和的含丙酮基的 IB-iBond，Kulzer，另一种是强水基的 PL，Adper Prompt L-Pop，3M ESPE）将标本分为三组（每组 n=12）。所有的腔均用 Filtek Z250（3M ESPE）树脂复合材料修复。对于每种粘接剂，将标本（每组 n=12，24 个修复腔）根据检查前的储存期进一步分为三组（每组 n=4，8 个腔）：24 小时、3 个月或 6 个月。用同样的方法制备另一组牙齿用于纳米渗漏模式评估。储存后，将标本置于 50%W/V 硝酸银溶液中 24 小时，然后在显影液中浸泡 8 小时。然后，标本沿颊舌方向进行切片、抛光，并通过扫描电子显微镜进行检查。对于纳米渗漏模式，标本的处理与纳米渗漏深度相同，不同之处在于抛光后用 10%EDTA 浸泡 5 秒。用银渗透百分比计算牙修复界面的总长度。数据采用双向方差分析、Kruskal-Wallis 和事后检验进行分析。
在 24 小时时，IB 的纳米渗漏深度最小，PL 的最大。对于储存的标本，所有粘接剂的纳米渗漏深度均无显著差异。测试的粘接剂记录了不同的纳米渗漏模式；然而，随着时间的推移，银沉积的强度和连续性增加。
在 24 小时时，纳米渗漏深度/模式随粘接剂的类型而变化；然而，在储存水后，所有粘接剂的性能相当。
