College of Food Science and Engineering, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 28 Xinong Road, Yangling, Xi'an, Shaanxi 712100, China.
Appl Biochem Biotechnol. 2011 Dec;165(7-8):1519-31. doi: 10.1007/s12010-011-9372-2. Epub 2011 Sep 23.
Strain M(438), deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-producing strain ever reported. In this paper, M(438) was identidied as Gluconacetobacter hansenii subsp. nov. on the basis of the results obtained by examining it phylogenetically, phenotypically, and physiologically-biochemically. Furthermore, the genetic diversity of strain M(438) and its initial strain was examined by amplified fragment length polymorphism. The results indicated that strain M(438) was a deletion mutant induced by HHP, and the only deleted sequence showed 99% identity with 24,917-24,723 bp in the genome sequence of Ga. hansenii ATCC23769, and the complement gene sequence was at 24,699-25,019 bp with local tag GXY_15142, which codes small multidrug resistance (SMR) protein. It can be inferred that SMR might be related to inhibiting BC production to a certain extent.
菌株 M(438)，保藏号为 CGMCC3917，从家庭自制醋中筛选出的产细菌纤维素（BC）的菌剂接种物中分离得到，然后通过高静压处理（HHP）诱导，具有比原始菌株高出三倍以上的 BC 生产能力。这是迄今为止报道的最高产 BC 的菌株。本文根据菌株 M(438)的系统发育、表型和生理生化特征的研究结果，将其鉴定为葡糖醋杆菌（Gluconacetobacter hansenii）的一个亚种。此外，还通过扩增片段长度多态性分析了菌株 M(438)及其原始菌株的遗传多样性。结果表明，菌株 M(438)是由 HHP 诱导的缺失突变体，缺失序列与 Ga. hansenii ATCC23769 基因组序列中的 24,917-24,723 bp 具有 99%的同源性，互补基因序列位于 24,699-25,019 bp 之间，带有局部标签 GXY_15142，该基因编码小多重耐药（SMR）蛋白。可以推断，SMR 可能在一定程度上与抑制 BC 生产有关。
