Shpakov A O
Tsitologiia. 2011;53(8):633-44.
The peptide strategy, a new direction of molecular endocrinology, includes the synthesis of peptides corresponding to functional regions of signal proteins, the use of the peptides for study of the molecular mechanisms of transduction of hormonal signal into cell ant the development of selective regulators of hormonal signaling systems on the basis of these peptides. The peptide strategy is used for study a wide spectrum of the proteins, components of signal systems, the proteins possessing the catalytic activity in particular, such as tyrosine kinases receptors, the enzymes generating the second messengers, serine/threonine protein kinase, phosphatases. In the first time in the review the data concerning the synthetic peptides, derivatives of the primary structure of proteins with the enzymatic activity, their application for study of the structural-functional organization and the molecular mechanisms of action of signal proteins, and the construction of regulators of fundamental cell processes on the basis of these peptides are analyzed and summarized.
肽策略是分子内分泌学的一个新方向，包括合成与信号蛋白功能区域相对应的肽，利用这些肽研究激素信号转导至细胞的分子机制，以及基于这些肽开发激素信号系统的选择性调节剂。肽策略用于研究广泛的蛋白质、信号系统的组成成分，特别是具有催化活性的蛋白质，如酪氨酸激酶受体、产生第二信使的酶、丝氨酸/苏氨酸蛋白激酶、磷酸酶。本文首次对有关合成肽、具有酶活性的蛋白质一级结构衍生物、它们在研究信号蛋白的结构功能组织和作用分子机制方面的应用，以及基于这些肽构建基本细胞过程调节剂的数据进行了分析和总结。
