Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China.
Talanta. 2011 Oct 15;85(5):2695-9. doi: 10.1016/j.talanta.2011.06.032. Epub 2011 Jun 17.
A novel fluorescent Au@SiO(2) nanocomposite, with average size of ca. 30 nm in the diameter, was prepared via a simple microemulsion method. Additionally, transmission electron microscopy (TEM), UV-Vis absorption spectra, Fourier transform infrared (FT-IR) spectra and fluorescence spectra were used to characterize this nanocomposite. This newly synthesized, silica-wrapped, gold nanocluster has the following advantages: good water solubility, exceptional biocompatibility, favorable surface properties and excellent fluorescence properties. Because of these advantages, a Au@SiO(2) nanocomposite is exceptionally suitable for biological applications. In this study, cellular imaging, as a form of biological application, has been fully investigated, and it was discovered, after covalent conjugation of folic acid (FA), that the nanocomposite effectively recognized over expressed folic acid receptors (FARs) on the HeLa cell's surface. Therefore, this fluorescent Au@SiO(2) nanocomposite could be used as a new fluorescent probe for selective biological imaging.
一种新型的荧光 Au@SiO(2) 纳米复合材料，其平均粒径约为 30nm，通过简单的微乳液法制备。此外，还使用透射电子显微镜 (TEM)、紫外-可见吸收光谱、傅里叶变换红外 (FT-IR) 光谱和荧光光谱对该纳米复合材料进行了表征。这种新合成的、被二氧化硅包裹的金纳米簇具有以下优点：良好的水溶性、出色的生物相容性、良好的表面性质和优异的荧光性质。由于这些优点，Au@SiO(2) 纳米复合材料非常适合生物应用。在这项研究中，作为生物应用的一种形式，细胞成像得到了充分的研究，并且发现在叶酸 (FA) 的共价偶联之后，纳米复合材料能够有效地识别 HeLa 细胞表面过表达的叶酸受体 (FARs)。因此，这种荧光 Au@SiO(2) 纳米复合材料可以用作选择性生物成像的新型荧光探针。
